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Recombinant Xenopus laevis Protein argonaute-2 (eif2c2), partial

  • 中文名称:
    Recombinant Xenopus laevis Protein argonaute-2(eif2c2) ,partial
  • 货号:
    CSB-YP718722XBE
  • 规格:
  • 来源:
    Yeast
  • 其他:
  • 中文名称:
    Recombinant Xenopus laevis Protein argonaute-2(eif2c2) ,partial
  • 货号:
    CSB-EP718722XBE
  • 规格:
  • 来源:
    E.coli
  • 其他:
  • 中文名称:
    Recombinant Xenopus laevis Protein argonaute-2(eif2c2) ,partial
  • 货号:
    CSB-EP718722XBE-B
  • 规格:
  • 来源:
    E.coli
  • 共轭:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名称:
    Recombinant Xenopus laevis Protein argonaute-2(eif2c2) ,partial
  • 货号:
    CSB-BP718722XBE
  • 规格:
  • 来源:
    Baculovirus
  • 其他:
  • 中文名称:
    Recombinant Xenopus laevis Protein argonaute-2(eif2c2) ,partial
  • 货号:
    CSB-MP718722XBE
  • 规格:
  • 来源:
    Mammalian cell
  • 其他:

产品详情

  • 纯度:
    >85% (SDS-PAGE)
  • 基因名:
    ago2
  • Uniprot No.:
  • 别名:
    ago2; eif2c2Protein argonaute-2; Argonaute2; EC 3.1.26.n2; Argonaute RISC catalytic component 2; Eukaryotic translation initiation factor 2C 2; eIF-2C 2; eIF2C 2; Protein slicer
  • 种属:
    Xenopus laevis (African clawed frog)
  • 蛋白长度:
    Partial
  • 蛋白标签:
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

产品评价

靶点详情

  • 功能:
    Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include ago2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by ago2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur.
  • 亚细胞定位:
    Cytoplasm, P-body.
  • 蛋白家族:
    Argonaute family, Ago subfamily
  • 数据库链接:

    KEGG: xla:446823

    UniGene: Xl.43287