Recombinant Human Melanoma-associated antigen 4 (MAGEA4) (Active)

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中文名称：

人MAGEA4重组蛋白
货号：

CSB-MP013330HU
规格：

￥828
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human MAGEA4 at 2 μg/ml can bind anti-MAGEA4 recombinant antibody, the EC₅₀ is 30.33-43.10 ng/mL. Biological Activity Assay
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纯度：

Greater than 90% as determined by SDS-PAGE.
内毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

Measured by its binding ability in a functional ELISA. Immobilized Human MAGEA4 at 2 μg/Ml can bind anti-MAGEA4 recombinant antibody, the EC₅₀ is 30.33-43.10 ng/mL.
基因名：

MAGEA4
Uniprot No.：

P43358
别名：

(Cancer/testis antigen 1.4) (CT1.4) (MAGE-4 antigen) (MAGE-41 antigen) (MAGE-X2 antigen)
分子结构：
种属：

Homo sapiens (Human)
蛋白长度：

Full Length
来源：

Mammalian cell
分子量：

36.7 kDa
表达区域：

1-317aa
氨基酸序列

MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESAGPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPASNTYTLVTCLGLSYDGLLGNNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQDWVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRIAYPSLREAALLEEEEGV
蛋白标签：

C-terminal 10xHis-tagged
产品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
产品描述：

The generation of the recombinant human melanoma-associated antigen 4 (MAGEA4) includes the fusion of the 10xHis-tag gene with all genes encoding the human MAGEA4, the cloning of the synthesized gene into the plasmid vector, the transfection of the cloned vector into the mammalian cells. This recombinant human MAGEA4 protein was quality tested in the purity, endotoxin level, and bioactivity. Its purity reached up to 90% measured by SDS-PAGE. The LAL method estimated the endotoxin content of this MAGEA4 protein less than 1.0 EU/ug. In the functional ELISA, this MAGEA4 protein can bind to the recombinant antibody against MAGEA4, with the EC₅₀ of 30.33-43.10 ng/mL.

MAGEA4 is up-regulated in various malignant tumors such as oral squamous cell carcinoma, non-small cell lung cancer, pancreatic cancer, and breast cancer. It is not expressed in adult normal tissues but in germ cells. MAGEA4 plays a dual role as a tumor suppressor and tumor promoter during tumorigenesis. As a tumor promoter, MAGEA4 is highly expressed in liver cancer cells and inhibits caspase-mediated apoptosis, reduces the sensitivity to chemotherapy, and ultimately reduces p53 activity, thereby inhibiting apoptosis. MACEA4 acts as a master regulator of Tregs development and function. Tregs are involved in tumor escape from the immune system. MAGEA4 in pancreatic cancer inhibits T cell proliferation, thus facilitating tumor cell survival. As a tumor-specific antigen, MAGEA4 is helpful to establish a new antitumor method.
Datasheet & COA：

Please contact us to get it.

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功能：

Regulates cell proliferation through the inhibition of cell cycle arrest at the G1 phase. Also negatively regulates p53-mediated apoptosis.
基因功能参考文献：
1. MAGEA4 was positively correlated with TWIST1, and its knockdown inhibited EMT in TE3 cells. PMID: 28653599
2. Because of the significant correlation of MAGEA4 and indices of poor prognosis, the role of this CTA may be confirmed in Esophageal squamous cell carcinoma (ESCC) aggressiveness and metastasis. Therefore, MAGEA4 may be a promising therapeutic candidate for suppressing ESCC aggressiveness PMID: 30197348
3. Study shows that whereas MAGEA4 expression by itself may be indicative of a poor prognosis in primary lung cancer, its prognostic value depends entirely on its subcellular localization and on the p53 status. The accumulation of nuclear MAGEA4 expression without p53 expression is significantly associated with poor survival, implying that MAGEA4 inhibits apoptosis and increases tumorigenesis. PMID: 29901069
4. MAGE-A4 depletion from MAGE-A4-expressing cancer cells destabilizes RAD18. PMID: 27377895
5. Our results support...the potential utility of MAGEA4 as an ancillary diagnostic marker for synovial sarcoma PMID: 28744588
6. some autoantibodies, such as anti-MAGEA4, anti-CTAG1 or anti-TP53 and their combinations could possibly contribute to the development of cancer early detection tests (not necessarily restricted to gastric cancer) when being combined with other markers. PMID: 27140836
7. Anti-MAGEA4 exhibited the highest sensitivity for detecting early stage colorectal cancer (CRC) and advanced adenoma. PMID: 26909861
8. High MAGE-A4 expression is associated with Lung Cancer. PMID: 27793776
9. Results found concomitant overexpression of MAGEA4 and TWIST1 in esophageal squamous cell carcinoma (ESCC). Also, it showed a correlation between MAGEA4 and TWIST1 expression, which was found as indirect binding of TWIST1 to the E-boxes in the MAGEA4 promoter revealing transcriptional upregulation of MAGEA4 by TWIST1. PMID: 27533647
10. Results support the potential utility of NY-ESO-1, PRAME, and MAGEA4 as targets for immunotherapy and as ancillary prognostic parameters in synovial sarcomas. PMID: 27993576
11. CTAs (MAGE-A4, NY-ESO-1, MAGE-A10) were more likely expressed in patients with squamous cell carcinoma of the lung and when CTAs combined with CD133, they can be better prognostic factors. PMID: 26191258
12. HLA class I loss in smokers or patients with the MAGE-A4 gene was a prognostic factors in non-small cell lung cancer . PMID: 23124547
13. Expression of MAGE-A4 may make immunotherapeutic intervention possible in selected patients with thyroid cancer. PMID: 24238058
14. Detection of MAGE-4 transcripts in blood may help to predict the prognosis and monitoring of the response of HCV-infected hepatocellular carcinoma patients to therapy PMID: 22653756
15. Mage-A4 expression and serum identification is and potential therapeutic target in estrogen receptor negative breast cancer. PMID: 23172894
16. MAGEA4 promotes growth by preventing cell cycle arrest and by inhibiting apoptosis mediated by the p53 transcriptional targets. PMID: 22842486
17. High MAGE-4 gene expression is associated with metastases in hepatitis C virus patients complicated by hepatocellular carcinoma. PMID: 21452042
18. MAGE-A4 is identified as a specific biomarker of esophageal squamous cell carcinoma with a possible oncogenic role contributing to tumor progression. PMID: 21613820
19. novel HLA-A2-restricted T cell epitope derived from MAGE-4 was identified PMID: 21815906
20. High MAGE-A4 gene expression is associated with head and neck squamous cell carcinoma PMID: 20715104
21. Report immunohistochemical expression of MAGE-A4 in renal oncocytoma and chromophobe renal cell carcinoma. PMID: 20591578
22. positive expression in patients with non-small cell lung cancer is associated with poorer overall survival PMID: 19545928
23. MAGE-4 protein may have a role in development of hepatocellular carcinogenesis in cirrhotic patients PMID: 11985796
24. MAGE-A4 expression in bladder transitional cell carcinoma. PMID: 12209610
25. strong MAGE-A4 expression and to a lesser degree NY-ESO-1 expression is characteristic of the vast majority of uterine carcinosarcomas and a major subset of papillary serous carcinomas PMID: 12209997
26. MAGE-A4 binds to gankyrin and suppresses its oncogenic activity. PMID: 12525503
27. a cleaved form of MAGE-A4 binds to Miz-1 and induces apoptosis PMID: 14739298
28. MAGE-A4(143-151) and SAGE(715-723) are HLA-A2402-restricted CTL epitopes PMID: 16061876
29. Expression may represent potential targets for cancer immunotherapy in patients with non small cell lung carcinoma. PMID: 16596224
30. Expressed in a large part of epithelial skin tumors with predominantly scattered immunoexpression pattern in organ-transplant recipients. PMID: 17214847
31. Its expression is significantly associated with prognostic factors in poor outcome of the non-small cell lung cancer. PMID: 18982744
32. Overexpression of MAGE-A4 is associated with bladder cancer. PMID: 19533752
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组织特异性：

Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
数据库链接：

HGNC: 6802

OMIM: 300175

KEGG: hsa:4103

STRING: 9606.ENSP00000276344

UniGene: Hs.37107

Recombinant Human Melanoma-associated antigen 4 (MAGEA4) (Active)

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