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  4. Recombinant Staphylococcus aureus Histidine protein kinase saeS (saeS), partial

Recombinant Staphylococcus aureus Histidine protein kinase saeS (saeS), partial

  • 中文名称：
    金黄色酿脓葡萄球菌saeS重组蛋白
  • 货号：
    CSB-YP643036FLF1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    金黄色酿脓葡萄球菌saeS重组蛋白
  • 货号：
    CSB-EP643036FLF1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    金黄色酿脓葡萄球菌saeS重组蛋白
  • 货号：
    CSB-EP643036FLF1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    金黄色酿脓葡萄球菌saeS重组蛋白
  • 货号：
    CSB-BP643036FLF1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    金黄色酿脓葡萄球菌saeS重组蛋白
  • 货号：
    CSB-MP643036FLF1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    saeS
  • Uniprot No.：
    Q2G2U1
  • 别名：
    saeS; SAOUHSC_00714; Histidine protein kinase SaeS; Sensor protein SaeS; Staphylococcus exoprotein expression protein S
  • 种属：
    Staphylococcus aureus (strain NCTC 8325)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

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saeS Proteins

靶点详情

  • 功能：
    Member of the two-component regulatory system SaeR/SaeS involved in the regulation of staphylococcal virulence factors in a strain-dependent fashion. Probably functions as a membrane-associated protein kinase that upon sensing the appropriate signal, autophosphorylates and in turn activates the cytosolic response regulator SaeR. SaeR/SaeS activates the expression of exoproteins involved in adhesion and invasion of host cells, including hemolysins (hla, hlb), coa, Dnase, spa and cell wall-associated proteins (emp, eap, fnbA). Acts probably downstream of the agr system in the regulatory cascade of virulence factors.
  • 基因功能参考文献：
    1. the crystal structure of the DNA-binding domain of SaeR (SaeR(DBD)) was solved at 2.5 A resolution. The structure reveals that SaeR(DBD) exists as a monomer and has the canonical winged helix-turn-helix module. [SaeR] PMID: 26249357
    2. Our results indicate that the function of the linker peptide in SaeS is a highly evolved feature with very optimized amino acid sequences PMID: 25849574
    3. The SaeS protein is the histidine kinase responsible for phosphorylation of the response regulator SaeR. RNA-Seq results indicated that sae regulates many genes that may affect biofilm formation PMID: 25853849
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 数据库链接：

    KEGG: sao:SAOUHSC_00714

    STRING: 93061.SAOUHSC_00714

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