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  4. Recombinant Schizosaccharomyces pombe Structure-specific endonuclease subunit slx4 (slx4)

Recombinant Schizosaccharomyces pombe Structure-specific endonuclease subunit slx4 (slx4)

  • 中文名称：
    庞贝裂殖酵母slx4重组蛋白
  • 货号：
    CSB-YP882212SXV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    庞贝裂殖酵母slx4重组蛋白
  • 货号：
    CSB-EP882212SXV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    庞贝裂殖酵母slx4重组蛋白
  • 货号：
    CSB-EP882212SXV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    庞贝裂殖酵母slx4重组蛋白
  • 货号：
    CSB-BP882212SXV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    庞贝裂殖酵母slx4重组蛋白
  • 货号：
    CSB-MP882212SXV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    slx4
  • Uniprot No.：
    Q9P6M0
  • 别名：
    slx4; SPAC688.06c; Structure-specific endonuclease subunit slx4; Synthetic lethal of unknown function protein 4
  • 种属：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-419
  • 氨基酸序列
    MSAEEYIEVS SSPDIFTDDD DMITIEPELN KNPKDCNSKR KRSVTECCEI RLITSKCDFE STQQLVHHNC TGHKVHEHNL NAVDEEDFDT ENLPLLFSSF SDNESDILEP DLNTRVAEDN DVLLSRYSKI KNSASCRNTF EHSAYHSNRE EISSSGFYYH RKPQLFEKSL EKLGNKSIEA NRSPLIKELC ESANSTENVC FSVSTVDEIQ QRHPSAGHSI DSTCQSNSFL EGDSATHKKK KTDNIKEFTS CEFNDRSRTL LNYAGYMDTN KNADNEAKSL KEKLENFPVE KLRAIAESYG FKSSDSKATL IKIVESCLDA IDSRSQSKKL GKETPHDYLI TSTKTVLEFD DIVTQTHRAI SQVVKQAKDN SVWIKILTYS AIDVEEFQLW LKRKNLNVSL DLIKSWCDKY GVLMKGSWH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulatory subunit of the slx1-slx4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for stem-loop (SL) and splayed arm Y structures. Introduces a single-strand cut in duplex DNA on the 3' side of a double-strand/single-strand junction with respect to the single-strand moving 3' to 5' away from the junction. Plays a critical role in maintaining the integrity of the ribosomal DNA (rDNA) loci, where it has a role in re-starting stalled replication forks. The complex initiates homologous recombination (HR) events, used to maintain rDNA copy number, in the rDNA repeats that are processed by a mechanism that requires rad22, but not rhp51. Has Holliday junction resolvase activity in vitro. Slx4 is required for efficient processing of DNA substrates.
  • 亚细胞定位：
    Nucleus, nucleolus.
  • 蛋白家族：
    SLX4 family
  • 数据库链接：

    KEGG: spo:SPAC688.06c

    STRING: 4896.SPAC688.06c.1

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