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  4. Recombinant Saccharomyces cerevisiae Elongation factor 1 alpha-like protein (HBS1), partial

Recombinant Saccharomyces cerevisiae Elongation factor 1 alpha-like protein (HBS1), partial

  • 中文名称：
    酿酒酵母HBS1重组蛋白
  • 货号：
    CSB-YP333731SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母HBS1重组蛋白
  • 货号：
    CSB-EP333731SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母HBS1重组蛋白
  • 货号：
    CSB-EP333731SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母HBS1重组蛋白
  • 货号：
    CSB-BP333731SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母HBS1重组蛋白
  • 货号：
    CSB-MP333731SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HBS1
  • Uniprot No.：
    P32769
  • 别名：
    HBS1; YKR084C; YKR404; Elongation factor 1 alpha-like protein
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in protein translation. Together with DOM34, may function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs.
  • 基因功能参考文献：
    1. analysis establishes the structural foundation for the recognition of aberrantly stalled 80S ribosomes by the Dom34.Hbs1.GTP complex during Dom34-mediated mRNA surveillance pathways PMID: 27995908
    2. Roles of dom34:hbs1 in nonstop protein clearance from translocators for normal organelle protein influx. PMID: 22981232
    3. Dom34:Hbs1 facilitates the decay of nonstop mRNAs from the 3' end by exosomes and is required for the complete degradation of nonstop mRNA decay intermediates. PMID: 22503425
    4. The close structural similarity of Dom34 and Hbs1 to eukaryotic release factors (eRFs) enabled the authors to propose a model for the ribosome-bound eRF1-eRF3 complex. PMID: 21623367
    5. findings show that Dom34:Hbs1 (two interacting factors genetically implicated in no-go decay) interacts with the ribosome to promote subunit dissociation and peptidyl-tRNA drop-off PMID: 20947765
    6. Dom34 has a role in an mRNA decay pathway called No-Go decay and the interaction of Dom34 with Hbs1 results in an increase in the affinity constant of Hbs1 for GTP but not GDP PMID: 18180287

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family
  • 数据库链接：

    KEGG: sce:YKR084C

    STRING: 4932.YKR084C

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