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  4. Recombinant Saccharomyces cerevisiae DNA topoisomerase 2-associated protein PAT1 (PAT1), partial

Recombinant Saccharomyces cerevisiae DNA topoisomerase 2-associated protein PAT1 (PAT1), partial

  • 中文名称：
    酿酒酵母PAT1重组蛋白
  • 货号：
    CSB-YP326562SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母PAT1重组蛋白
  • 货号：
    CSB-EP326562SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母PAT1重组蛋白
  • 货号：
    CSB-EP326562SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母PAT1重组蛋白
  • 货号：
    CSB-BP326562SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母PAT1重组蛋白
  • 货号：
    CSB-MP326562SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PAT1
  • Uniprot No.：
    P25644
  • 别名：
    PAT1; MRT1; YCR077C; YCR77C; DNA topoisomerase 2-associated protein PAT1; Decapping activator and translational repressor PAT1; Topoisomerase II-associated protein PAT1; mRNA turnover protein 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Activator of decapping that functions as a general and active mechanism of translational repression and required for P-body formation. First decay factor recruited to mRNA, at a time when the mRNA is still associated with translation factors. Subsequently, PAT1 recruits the hepta-heterodimer LSM1-LSM7 complex to P-bodies. In association with the LSM1-LSM7 complex, stabilizes the 3' terminus of mRNAs. This association is also required for mosaic virus genomic RNA translation. Modulates the rates of mRNA-decapping that occur following deadenylation. Might be required for promoting the formation or the stabilization of the preinitiation translation complexes. Required for 40S ribosomal subunit joining to capped and/or polyadenylated mRNA. With other P-body components, enhances the formation of retrotransposition-competent Ty1 virus-like particles. Necessary for accurate chromosome transmission during cell division.
  • 基因功能参考文献：
    1. the studies presented here identify a set of residues at the very C-terminal end of Lsm1 to be functionally important and suggest that these residues support the function of the Lsm1-7-Pat1 complex by facilitating RNA binding either directly or indirectly PMID: 27434131
    2. Pat1 affects the structure of CEN chromatin and protects Cse4 from Psh1-mediated ubiquitination for faithful chromosome segregation. PMID: 25833709
    3. The mRNA fate modulators Dhh1 and Pat1 redundantly stimulate mRNA decay, but both factors are required for poly(A) tail-independent translation repression by tethered TNRC6A. PMID: 25657010
    4. Pat1 C-terminal domain displays two conserved regions involved in decapping factor recruitment PMID: 24830408
    5. The middle domain of Pat1 is essential for the interaction of Pat1 with the Lsm1-7 complex in vivo. PMID: 25035297
    6. The Pat1 C-terminal domain interacting with the C-terminal helix of Lsm2 and the N-terminal helix of Lsm3 extended RNA-binding surface provides a possible molecular explanation for the preferred binding of the Lsm1-7-Pat1 complex to longer RNAs. PMID: 24247251
    7. our data have uncovered a novel role for Pat1 in maintaining the structural integrity of centromeric chromatin to facilitate faithful chromosome segregation and proper kinetochore function. PMID: 23893485
    8. These data provide the first evidence that Dhh1 is required for Pat1 nuclear relocation after UV stress. PMID: 23847025
    9. The results suggest a model for how Dhh1-containing messenger ribonucleoprotein particles might be remodeled upon Pat1 and Edc3 binding. PMID: 23851565
    10. LSm1, Pat1, and Dhh1 control the RNA3/RNA1 ratio of Flock House virus RNAs. PMID: 23536653
    11. Pull-down experiments reveal that the intracellular binding partners of Pat1 change as cells undergo the diauxic growth shift. PMID: 21712243
    12. inhibition of translation initiation and induction of P-body formation by overproduction of Dhh1 or Pat1 cause tRNA nuclear accumulation in nutrient-replete conditions PMID: 21398402
    13. The overexpression of Pat1p causes translational repression, P body formation, and arrests cell growth. PMID: 16179257
    14. The Lsm1p-7p-Pat1p complex protects mRNAs by binding at/near the 3'end and distinguishes between oligoadenyLated and polyadenylated RNA. PMID: 17513695
    15. These results indicate that Pat1 is an RNA binding protein and a multidomain protein that functions at multiple stages in the process of translation repression and mRNA decapping PMID: 18086885
    16. These results underscore the importance of Lsm1p-7p-Pat1p complex-mRNA interaction for mRNA decay in vivo and imply that the oligo(A) tail mediated enhancement of such interaction is crucial in that process. PMID: 18719247

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cytoplasm, P-body.
  • 蛋白家族：
    PAT1 family
  • 数据库链接：

    KEGG: sce:YCR077C

    STRING: 4932.YCR077C

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