Recombinant Saccharomyces cerevisiae Cysteine desulfurase, mitochondrial (NFS1)
-
中文名称:酿酒酵母NFS1重组蛋白
-
货号:CSB-YP326540SVG
-
规格:
-
来源:Yeast
-
其他:
-
中文名称:酿酒酵母NFS1重组蛋白
-
货号:CSB-EP326540SVG
-
规格:
-
来源:E.coli
-
其他:
-
中文名称:酿酒酵母NFS1重组蛋白
-
货号:CSB-EP326540SVG-B
-
规格:
-
来源:E.coli
-
共轭:Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
-
其他:
-
中文名称:酿酒酵母NFS1重组蛋白
-
货号:CSB-BP326540SVG
-
规格:
-
来源:Baculovirus
-
其他:
-
中文名称:酿酒酵母NFS1重组蛋白
-
货号:CSB-MP326540SVG
-
规格:
-
来源:Mammalian cell
-
其他:
产品详情
-
纯度:>85% (SDS-PAGE)
-
基因名:
-
Uniprot No.:
-
别名:NFS1; SPL1; YCL017C; Cysteine desulfurase; mitochondrial; EC 2.8.1.7; tRNA-splicing protein SPL1
-
种属:Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
-
蛋白长度:Full Length of Mature Protein
-
表达区域:34-497
-
氨基酸序列YSPPAAG VKLDDNFSLE THTDIQAAAK AQASARASAS GTTPDAVVAS GSTAMSHAYQ ENTGFGTRPI YLDMQATTPT DPRVLDTMLK FYTGLYGNPH SNTHSYGWET NTAVENARAH VAKMINADPK EIIFTSGATE SNNMVLKGVP RFYKKTKKHI ITTRTEHKCV LEAARAMMKE GFEVTFLNVD DQGLIDLKEL EDAIRPDTCL VSVMAVNNEI GVIQPIKEIG AICRKNKIYF HTDAAQAYGK IHIDVNEMNI DLLSISSHKI YGPKGIGAIY VRRRPRVRLE PLLSGGGQER GLRSGTLAPP LVAGFGEAAR LMKKEFDNDQ AHIKRLSDKL VKGLLSAEHT TLNGSPDHRY PGCVNVSFAY VEGESLLMAL RDIALSSGSA CTSASLEPSY VLHALGKDDA LAHSSIRFGI GRFSTEEEVD YVVKAVSDRV KFLRELSPLW EMVQEGIDLN SIKWSGH
-
蛋白标签:Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
产品提供形式:Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
复溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
-
储存条件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
-
保质期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
货期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
-
注意事项:Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
-
Datasheet :Please contact us to get it.
靶点详情
-
功能:Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. Plays a role in both tRNA-processing and mitochondrial metabolism. Involved in the 2-thio-modification of both 5-carboxymethylaminomethyl-2-thiouridine in mitochondrial tRNAs and 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) in cytoplasmic tRNAs.
-
基因功能参考文献:
- Compared with wild-type mitochondria, yck2 mitochondria revealed slower persulfide formation on Nfs1 consistent with a role of Yck2 in regulating mitochondrial cysteine desulfurase activity. PMID: 28941588
- Isu is a substrate of the Lon-type protease and that it is protected from degradation by Nfs1. Nfs1 variants known to be defective in interaction with Isu were also defective in protecting Isu from degradation. PMID: 26842892
- Mutual exclusivity of cluster assembly factor (Nfs1/Yfh1) and cluster transfer factor (Jac1/Ssq1) binding to Isu has functional consequences for the transition from the assembly process to the transfer process during biogenesis of FeS cluster proteins. PMID: 25228696
- The data presented here show that the Isu1 suppressor mimics the frataxin effects on Nfs1, explaining the bypassing activity. PMID: 24217246
- results show that Nfs1 adopts a higher oligomeric state in the Isd11-bound form compared to that in the free form PMID: 24045011
- Competition between Jac1 and Nfs1 for Isu binding plays an important role in transitioning the iron/Sulfur cluster biogenesis machinery. PMID: 23946486
- Thus the Nfs1p-bound persulfide as described here represents a precursor en route to Fe-S cluster synthesis in mitochondria. PMID: 22813754
- Whereas Nfs1p by itself was inactive, the Nfs1p.Isd11p complex formed persulfide and was active as a cysteine desulfurase. In the absence of Isd11p, Nfs1p was able to bind the substrate cysteine but failed to form a persulfide. PMID: 22928949
- Isd11 acts together with Nfs1 in an early step in the biogenesis of Fe/S proteins. PMID: 16341090
- Results are consistent with the existence of a mechanism to increase the stability of Isu, and thus its level, that is dependent on the presence of the cysteine desulfurase Nfs1. PMID: 18843040
显示更多
收起更多
-
亚细胞定位:Mitochondrion.
-
蛋白家族:Class-V pyridoxal-phosphate-dependent aminotransferase family, NifS/IscS subfamily
-
数据库链接:
KEGG: sce:YCL017C
STRING: 4932.YCL017C
Most popular with customers
-
Recombinant Human Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Human Tumor necrosis factor receptor superfamily member 8 (TNFRSF8), partial (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Human Poliovirus receptor (PVR) (I340M), partial (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Human HLA class II histocompatibility antigen gamma chain (CD74), partial (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Rat Microtubule-associated protein tau (Mapt) (Active)
Express system: Mammalian cell
Species: Rattus norvegicus (Rat)
-
Express system: Mammalian cell
Species: Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
-
Recombinant Human Pancreatic adenocarcinoma up-regulated factor (ZG16B) (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Human IL12B&IL12A Heterodimer Protein (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)