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  4. Recombinant Rat Rho-associated protein kinase 2 (Rock2), partial

Recombinant Rat Rho-associated protein kinase 2 (Rock2), partial

  • 中文名称：
    大鼠Rock2重组蛋白
  • 货号：
    CSB-YP720322RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Rock2重组蛋白
  • 货号：
    CSB-EP720322RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Rock2重组蛋白
  • 货号：
    CSB-EP720322RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Rock2重组蛋白
  • 货号：
    CSB-BP720322RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Rock2重组蛋白
  • 货号：
    CSB-MP720322RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rock2
  • Uniprot No.：
    Q62868
  • 别名：
    Rock2; Rho-associated protein kinase 2; EC 2.7.11.1; Rho-associated; coiled-coil-containing protein kinase 2; Rho-associated; coiled-coil-containing protein kinase II; ROCK-II; RhoA-binding kinase 2; p150 ROK-alpha; ROKalpha; p164 ROCK-2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays a role in placental homeostasis during the perinatal period. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
  • 基因功能参考文献：
    1. The findings suggest that miRNA-139-5p is capable of significantly influencing the phenotypic tonicity of the internal anal sphincter smooth muscle via ROCK2. PMID: 28469189
    2. acute hypobaric hypoxia augments ROCK2 protein level and activity PMID: 28657365
    3. We conclude that normal pregnancy suppresses ROCKs, which contributes to the low contractility during pregnancy; the RhoA/ROCK signalling pathway might be a potential target for the development of new tocolytic agents. PMID: 28820400
    4. High ROCK-II expression is associated with Diabetes-Induced Vascular Dysfunction. PMID: 23826343
    5. Panax notoginseng saponins provide neuroprotective effects in a rat model of cerebral ischemia and SH-SY5Y cells exposed to oxygen/glucose deprivation injury by inhibiting the overexpression of NgR1, RhoA, and ROCK2. PMID: 27288754
    6. Rho-Kinase inhibitor fasudil inhibited high glucose-induced apoptosis in rat H9c2 cells through activating autophagy. PMID: 27333569
    7. Increased contraction in the gastric smooth muscle of male rats is attributable to greater RhoA/ROCK activation independent of differences in the expression of ROCK isoforms or total RhoA. PMID: 26391686
    8. ROCK2 regulates contractile force and Rac1 activity at the leading edge of migratory cells and the spine head of neurons. PMID: 26169356
    9. Results demonstrate a clear role for ROCK in the development of LV dysfunction and impaired actin-myosin cross-bridge dynamics in early diabetic cardiomyopathy. PMID: 26194354
    10. Tetramethylpyrazine protects against acute lung injury by affecting rhoA/ROCK2 signaling. PMID: 26191148
    11. Rho kinase expression in the femoral artery during hindlimb unweighting and Rho kinase role in vasoconstriction in femoral arteries PMID: 25761652
    12. ROCK-2 inhibition restores ET-1-mediated NO production after the LPS pretreatment, in part, through an increase in actin depolymerization PMID: 25243430
    13. these results described a novel mechanism in atherosclerosis through PDGF-BB signaling in VSMC, in which MMP-2 expression is induced via extracellular signal-regulated kinases and p38 mitogen-activated protein kinase phosphorylation, as well as ROCK. PMID: 24792035
    14. The gene therapy with lentiviral vector-based siRNA-targeting ROCK2 mRNA can significantly improve erectile function mainly by directly inhibiting ROCK2 pathway in the SHR PMID: 25260465
    15. Over-activation of ROCK2 contributes to diabetic cardiomyopathy by multiple mechanisms, including direct phosphorylation and activation of protein kinase C beta II and interference with the PDK-1-mediated phosphorylation and activation of AKT. PMID: 24466133
    16. Characterize ROCK2 as a specific therapeutic target in neurodegenerative diseases and demonstrate new downstream effects of ROCK2 including axonal degeneration, apoptosis and autophagy. PMID: 24832597
    17. Suggest pantoprazole may impair gastric and lower sphincter muscle, exacerbating esophageal reflux via ROCK2 inhibtion. PMID: 24699328
    18. Downregulation of the expression of active-RhoA, ROCK1 and ROCK2 by Y-39983 promotes axonal regeneration. PMID: 23258382
    19. RhoA translocation but not ROCK-2 expression markedly increased by hyperosmolar glucose solution; the results may indicate that hyperosmolarity could induce vasoconstriction through Rho/Rho-kinase signalling PMID: 22136148
    20. Kamolonol suppresses angiotensin II-induced stress fiber formation and cellular hypertrophy through inhibition of Rho-associated kinase 2 activity PMID: 23891689
    21. ROCK2 is upregulated in vascular smooth muscle of diabetic aorta. PMID: 23530857
    22. These results demonstrated that ROCK1 and ROCK2 could promote VSMC proliferation through ERK nuclear translocation, regulating the expression of PCNA and cyclin D1 protein. PMID: 23402758
    23. Estrogens could up-regulate ROCK-2 in the rat coronary vascular endothelium through GPER1 and EGFR transactivation. PMID: 23641788
    24. Spironolactone lowers portal hypertension by improvement of liver fibrosis and inhibition of intrahepatic vasoconstriction via down-regulating ROCK-2 activity and activating NO/PKG pathway PMID: 22479572
    25. The rhoA/rhoA-associated kinase pathway is a key regulator of uterine activation during labor and may be a useful target for the prevention of spontaneous preterm birth. PMID: 22727353
    26. Hydroxyfasudil-mediated inhibition of ROCK1 and ROCK2 improves kidney function in rat renal acute ischemia-reperfusion injury. PMID: 22031832
    27. aldosterone induces contraction of activated hepatic stellate cells through the activation of the RhoA/ROCK-2 signaling pathway PMID: 21545816
    28. results provide evidence that depolymerization of the cortical actin cytoskeleton via inhibition of RhoA or its effector ROCKII favors the recruitment of V-ATPase from the cytosolic compartment into the apical membrane in epididymal clear cells PMID: 21411727
    29. ROCK-II may be dominant in enhancing survival of pulmonary arterial smooth muscle after rescue treatment with a Rho-kinase inhibitor in rats with chronic pulmonary hypertension. PMID: 20889845
    30. Treatment of urethral smooth muscle cells with estrogen, raloxifene or levormeloxifene resulted in decreased expression of Rock-II in a dosage-dependent manner. PMID: 20970835
    31. RhoA and Rho kinase-dependent phosphorylation of moesin at Thr-558 in hippocampal neuronal cells by glutamate PMID: 11867620
    32. Interferes with constriction induced by distinct signaling pathways in renal vessels without affecting F-actin structure. PMID: 12506136
    33. Angiotensin II, KCl, and myogenic constriction of afferent arterioles require calcium entry through voltage-dependent calcium channels. Interaction between Rho/Rho-kinase and calcium entry may determine afferent arteriolar tone induced by these stimuli. PMID: 12902637
    34. Chronic hypoxia mediates a shift in NO signaling to promote pulmonary vascular smooth muscle calcium desensitization through inhibition of RhoA/ROK. PMID: 15310556
    35. An enhanced role for the ROK pathway in alpha1-adrenergic vasoconstriction in metabolic syndrome is reported. PMID: 16141308
    36. Increased RhoA/Rho-kinase signaling is a major factor in the pathogenesis of age-associated erectile dysfunction and the mechanism involves increased penile smooth muscle contractility through inhibition of myosin light chain phosphatase. PMID: 16396994
    37. Two rho kinases contribute to fibronectin matrix assembly in a different manner and cortical myosin II-driven contractility, but not stress fibers, may be critical in this activity. PMID: 17065553
    38. ROCK activation occurs specifically during early myocardial reperfusion and contributes to irreversible myocardial damage. PMID: 17220176
    39. RhoA/ROCK play a critical role in maintenance of spontaneous tone in the internal anal sphincter smooth muscle via inhibition of myosin light chain phosphatase. PMID: 17379756
    40. both PKC and Rho/Rho-kinase mediate the heightened pulmonary vascular response to ET-1 in hypertensive pulmonary arterial smooth muscle PMID: 17468135
    41. Thus ET-1-mediated signaling in aorta and sMA is altered by IH-C but is dependent on different second messenger systems in small vs. large arteries. PMID: 17720771
    42. Rho-kinase activation with resultant eNOS down-regulation is substantially involved in the pathogenesis of portal hypertension in secondary biliary cirrhosis. PMID: 18167063
    43. chronic hypoxia augments Ca(2+) sensitization through ROS-dependent activation of RhoA/ROK signaling in pulmonary vascular smooth muscle PMID: 18621909
    44. lipid membrane binding properties of the PH-C1 tandem of rho kinases PMID: 18640982
    45. ROCK1 and ROCK2 regulated myosin light chain phosphatase. ROCK isoforms had distinct and opposing effects on vascular smooth muscle cell (VSMC) morphology and ROCK2, but not ROCK1, had a predominant role in VSMC contractility. PMID: 19131646
    46. ROCKII is regulated by localization to specific membrane compartments and its novel binding partner, Dynamin1. PMID: 19222995
    47. Data indicate that RhoA/Rok is involved in substratum-mediated regulation of insulin signaling in MECs, and under the conditions where proper adhesion to BM is missing, insulin-mediated cellular differentiation and survival would be defective. PMID: 19391109
    48. 15-hydroxyeicosatetraenoic acid protects pulmonary artery smooth muscle cells from apoptosis, contributing to pulmonary vascular medial thickening, and the effect is, at least in part, mediated via the ROCK pathway. PMID: 19746421

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  • 相关疾病：
    May play a role in hypertension. ROCK-inhibitors lower the blood pressure in spontaneous hypertensive, renal hypertensive and deoxycorticosterone acetate-induced hypertensive rats, but not in normal rats.
  • 亚细胞定位：
    Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Cytoplasmic, and associated with actin microfilaments and the plasma membrane.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family
  • 组织特异性：
    Highly expressed in brain, lung, liver, skeletal muscle, kidney and testis.
  • 数据库链接：

    KEGG: rno:25537

    STRING: 10116.ENSRNOP00000006403

    UniGene: Rn.88642

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