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  4. Recombinant Rat Rho-associated protein kinase 1 (Rock1), partial

Recombinant Rat Rho-associated protein kinase 1 (Rock1), partial

  • 中文名称：
    大鼠Rock1重组蛋白
  • 货号：
    CSB-YP717402RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Rock1重组蛋白
  • 货号：
    CSB-EP717402RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Rock1重组蛋白
  • 货号：
    CSB-EP717402RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Rock1重组蛋白
  • 货号：
    CSB-BP717402RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Rock1重组蛋白
  • 货号：
    CSB-MP717402RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rock1
  • Uniprot No.：
    Q63644
  • 别名：
    Rock1; Ac2-154; Rho-associated protein kinase 1; EC 2.7.11.1; Liver regeneration-related protein LRRG199; Rho-associated; coiled-coil-containing protein kinase 1; Rho-associated; coiled-coil-containing protein kinase I; ROCK-I; p150 RhoA-binding kinase ROK beta; p160 ROCK-1; p160ROCK
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles.
  • 基因功能参考文献：
    1. These findings reveal a novel regulatory mechanism of ROCK1/p53/NOXA signaling in modulating cardiomyocyte apoptosis in vitro and maternal diabetes-induced congenital heart defects in vivo. PMID: 28131915
    2. RhoA/ROCK pathway plays a role in adhesion molecule expression and inflammatory cell infiltration in glomerular endothelial cells induced by advanced glycation end products PMID: 28054559
    3. The findings indicate that upregulation of the RhoA/ROCK pathway is significantly associated with cardiac hypertrophy-related Ca2+ dysregulation and suggest that ROCK inhibition prevents hypertrophic heart failure. PMID: 29029794
    4. High ROCK1 expression is associated with Diabetes-Induced Vascular Dysfunction. PMID: 23826343
    5. excessive ROS derived from NADPH oxidase and mitochondria coordinate with RhoA/ROCK activation in a form of positive reciprocal relationship to induce endothelial dysfunction through disturbing endothelium-dependent NO signaling upon IS stimulation in chronic kidney disease status PMID: 28277985
    6. ET-1-ROCK interactions contribute to decreased alveolar and vascular growth. PMID: 27760762
    7. We found that a rounded shape with high cortical tension depends on MLCK and ROCK, whereas in cells with an elongated shape, MLCK is the primary protein responsible for cell spreading. Further, in cells with a slow and directionally persistent motility, MLCK predominates, while rapid and erratic movement is ROCK-dependent PMID: 28300565
    8. alpha-catulin has a unique function in co-localization with vimentin filaments that contributes to vascular endothelial cells migration via a pathway that may involve ROCK signaling. PMID: 26377600
    9. this study shows that paeoniflorin liver protective effect in non-alcoholic steatohepatitis is associated with inhibition of ROCK signaling pathway PMID: 27351828
    10. Lysophosphatidic acid and its receptor LPAR1, acting through the Rho-ROCK pathway, regulate P2X3 receptor in the development of both mechanical and spontaneous pain in bone cancer. PMID: 27094551
    11. The present results discourage the treatment of suspensions of dopaminergic precursors with ROCK inhibitors in the pregrafting period. PMID: 27075764
    12. These results suggest that Eph/ephrin reverse signalling negatively regulates axonal outgrowth and branching through RhoA/ROCK pathway in hippocampal neurons PMID: 25922200
    13. Sp6 positively regulates Rock1 transcription by direct binding to the Rock1 promoter region from -206 to -150, which functionally distinct from Sp1 PMID: 25264049
    14. The findings of this study suggested that a dysregulation of RhoA-ROCK activity by chronic stress could potentially underlie spine loss in hippocampal neurons. PMID: 26010004
    15. This report shows that Cx43 participates in the regulation of vascular permeability in sepsis and that the mechanism is related to the Rock1-MLC20 phosphorylation pathway. PMID: 26342084
    16. ROCK1 forms the stable actomyosin filament bundles that initiate front-back and dendritic spine polarity. PMID: 26169356
    17. We thus conclude that Ect2 is involved in annexin V-induced rat Leydig cell proliferation through the RhoA/ROCK pathway PMID: 25807302
    18. afadin mediates RhoA/ROCK signaling and contributes to the formation of actin stress fibers in podocyte cells PMID: 25712270
    19. These results indicated that klotho gene delivery ameliorated renal hypertrophy and fibrosis in diabetic rats, possibly by suppressing the ROCK signaling pathway PMID: 25695625
    20. Asymmetric dimethylarginine inducing vascular smooth muscle cells (VSMCs)migration via Rho/ROCK signal pathway is due to its effect on the VSMCs phenotype change. PMID: 25121106
    21. ROCK1 mediates the effects of miR-135a in tendon stem/progenitor cell. PMID: 25460182
    22. rhoA/rho-kinase and sGC/cGMP/PKG pathways play pivotal roles in cerebral vasospasm after subarachnoid hemorrhage. PMID: 24982890
    23. results of the present study suggest that LPA increases BBB permeability, which may be due to the Rho/ROCK signaling pathway PMID: 24398620
    24. Chronic dietary menthol blunted mesenteric arterial constriction and lowered blood pressure in genetic hypertensive rats via inhibition of RhoA/Rho kinase expression and activity in the vivo study. PMID: 24637663
    25. The regulation of vasopressin-induced Ca(2+) entry by ROCK in aorta could involve the myosin cytoskeleton and could be prevented by the downregulation of MLCK in VSMC. PMID: 22883550
    26. Data suggest that some of the beneficial effects of ROCK inhibitors in hypertension could be attributed to the mitigation of tumor necrosis factor alpha (TNF-alpha)-induced reduction in NOS3 expression. PMID: 22566503
    27. activated ROCK1 can promote vascular smooth muscle cell migration through facilitating phosphorylation and nuclear translocation of ERK protein. PMID: 22215561
    28. Hyper-phosphorylation of profilin2a is the molecular link between SMN and the ROCK pathway repressing neurite outgrowth in neuronal cells. PMID: 21920940
    29. Endogenous ROCK activity is necessary for the normal performance of motor output commands because it maintains afferent synaptic strength, by stabilizing the size of the readily releasable pool of synaptic vesicles. PMID: 22219271
    30. RhoK-mediated enhancement of myofilament Ca(2+) sensitivity is coupled with the alpha(1)-adrenergic vasoconstriction in penile arteries from prediabetic obese Zucker rats. PMID: 21297020
    31. The activation of RhoA/Rho-kinase signal pathway, stimulated by AT(1) receptor, plays an important role in the collar-induced the change of vascular contractility and reactivity PMID: 19942308
    32. renal nitric oxide system, Rho-kinase and Akt may contribute to the antihypertensive and renoprotective effects of statins PMID: 20724941
    33. Treatment of urethral smooth muscle cells with estrogen, raloxifene or levormeloxifene resulted in decreased expression of Rock-I in a dosage-dependent manner. PMID: 20970835
    34. Breviscapine was shown to prevent hypoxic pulmonary hypertension and decrease Rho-kinase. PMID: 19080536
    35. Data show that in vitro, the co-application of the Cdk5 inhibitor, Indolinone A, and the ROCK inhibitor, Y-27632, potentiated the survival-promoting effect of either substance alone on retinal ganglion cells. PMID: 19782753
    36. These results suggest that pulmonary endothelial dysfunction and activation of Rho kinase may contribute to the potentiation of vasoconstriction and hypoxemia in the pulmonary embolism rats. PMID: 19915157
    37. the anti-hypertrophic effects of NO are due, in part, to cGMP-dependent inhibition of the RhoA-ROCK-cofilin signalling pathway PMID: 19799911
    38. MEK is an effector of v-Src-induced cytoskeleton disruption, participating in v-Src-induced antagonism of the cellular function of Rho PMID: 12011049
    39. The ability of RhoA to increase monocyte adhesion and migration across endothelial cells was found to be mediated by its down-stream effector Rho kinase PMID: 14634067
    40. results demonstrate a role for the Rho-ROCK pathway in hippocampus-dependent spatial memory. (p160ROCK) PMID: 15336547
    41. Rho and Rho kinase are an important component of PTH signaling in osteoblastic cells PMID: 15476589
    42. Hypoxia increased cell stiffness and traction forces by a mechanism that was dependent on the activation of Rho kinase PMID: 15857906
    43. the Rho-kinase pathway is one potential upstream regulator of cardiac inflammatory response to burn injury PMID: 15988321
    44. results suggest Rho kinase-mediated sustained vasoconstriction plays an important role in established pulmonary hypertension (PH) & RhoA/Rho kinase signaling contributes significantly to development of PH & lung dysplasia in mild hypoxia-exposed rats PMID: 16322374
    45. Results suggest that ethanol-exposed astrocytes undergo apoptosis by anoikis and also that the RhoA/ROCK-I/MLC pathway participates in this process. PMID: 16390872
    46. ROCK regulates the phosphorylation of myosin light chain just behind the invading margin of tumor cells. PMID: 16890527
    47. These data highlight the role of ROCK in multiple processes in the execution phase of apoptosis, and suggest that ROCK1 plays an important role in controlling the outcome of apoptosis, that is, preparation of corpses for phagocytosis. PMID: 16904666
    48. These findings suggest that the activation of ROCK is involved in force development and CPI-17 phosphorylation induced by ET-1 stimulation in rat bronchial smooth muscle PMID: 17071121
    49. Involvement of Rho/ROCK in distinct morphogenetic processes necessary for kidney development and Rho/ROCK provides a potential mechanism to regulate overall branching patterns, nephron formation, and thus, kidney architecture. PMID: 17177859
    50. Small GTPases like RhoA along with their downstream effectors like ROCK I, ROCK II and Citron Kinase show a developmental stage specific expression in heart, with high levels being expressed in cardiomyocytes only at stages when cytokinesis still occurs. PMID: 17316608

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  • 相关疾病：
    May play a role in hypertension. Rock1-inhibitors lower the blood pressure in spontaneous hypertensive, renal hypertensive and deoxycorticosterone acetate-induced hypertensive rats, but not in normal rats.
  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family
  • 组织特异性：
    Highly expressed in brain, spleen, lung, liver, skeletal muscle, kidney and testis.
  • 数据库链接：

    KEGG: rno:81762

    STRING: 10116.ENSRNOP00000047378

    UniGene: Rn.89756

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