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  4. Recombinant Mouse Rho-associated protein kinase 1 (Rock1), partial

Recombinant Mouse Rho-associated protein kinase 1 (Rock1), partial

  • 中文名称：
    小鼠Rock1重组蛋白
  • 货号：
    CSB-YP020058MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rock1重组蛋白
  • 货号：
    CSB-EP020058MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rock1重组蛋白
  • 货号：
    CSB-EP020058MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rock1重组蛋白
  • 货号：
    CSB-BP020058MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rock1重组蛋白
  • 货号：
    CSB-MP020058MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rock1
  • Uniprot No.：
    P70335
  • 别名：
    Rock1; Rho-associated protein kinase 1; EC 2.7.11.1; Rho-associated; coiled-coil-containing protein kinase 1; Rho-associated; coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles.
  • 基因功能参考文献：
    1. by differentially affecting CyPA and Bsg expressions, ROCK1 protects and ROCK2 jeopardizes the heart from pressure-overload heart failure with postcapillary pulmonary hypertension, for which celastrol may be a promising agent. PMID: 29987023
    2. Data indicate that Rho-associated protein kinase (ROCK)-dependent intermittent T cell migration regulates tissue-sampling during acute lung injury. PMID: 29044117
    3. findings are the first to show that astrocytes exposed to MeHg showed increased cleavage/activation of ROCK-1, which was independent of the small GTPase RhoA PMID: 29428217
    4. the transcription of Rho-associated coiled-coil containing protein kinase 1 (ROCK1), which phosphorylates Drp1 at Ser616, was shown by luciferase assay to be directly regulated by FOXO1. These findings suggested that FOXO1 is critical to preserve mitochondrial quantity and function in ECs, and FOXO1 may serve as a therapeutic target for microvascular complications of diabetes. PMID: 29654945
    5. A specific inhibitor of ROCK, Y-27632, was used to examine the role of ROCK in mouse colitis models. ROCK1 and ROCK2 were silenced respectively using RNA interference in Caco-2 cells. The expression of tight junction proteins and the downstream molecules of ROCK were assessed PMID: 29115372
    6. GTP-RhoA and ROCK1 expression levels were markedly increased in a time-dependent manner in the ears and lungs of mice treated with penicillin. PMID: 27619816
    7. Irisin replenishment in mCaROCK1 mice partially reversed insulin resistance. PMID: 27411515
    8. MicroRNA-146a suppresses ROCK1 allowing hyperphosphorylation of tau in Alzheimer's disease PMID: 27221467
    9. Fasudil exhibited protective effects on smoke exposure induced cognitive deficits which might involve with the regulation of Rho/ROCK/NF-kappaB pathways. PMID: 27791202
    10. Dexamethasone up-regulates ROCK1/2 activity promoting migration, invasion and metastasis of melanoma cells. PMID: 28923399
    11. mDia induces circumferential actin filaments around the edge of the synaptic cleft, which contract the presynaptic terminals in a ROCK-dependent manner. PMID: 27880913
    12. SNRK in cardiomyocytes is responsible for maintaining cardiac metabolic homeostasis, which is mediated in part by ROCK, and alteration of this homeostasis influences cardiac function in the adult heart. PMID: 27780848
    13. we uncovered cell state plasticity and adhesion dynamics regulated by Ror2, which influenced Ras Homology Family Member A (RhoA) and Rho-Associated Coiled-Coil Kinase 1 (ROCK1) activity downstream of Dishevelled-2 (Dvl2). PMID: 28650466
    14. Data indicate that oxidative stress in diabetes causes a decrease in miR-133a expression leading to an increase in RhoA/Rho kinase pathway and muscle contraction. PMID: 28678840
    15. that reduction of ROCK1 diminishes amyloid-beta levels by enhancing Amyloid beta-Protein Precursor protein degradation PMID: 27246255
    16. the cross-talk between canonical and non-canonical signaling pathways of Wnt3A, which induces GSK-3beta phosphorylation and beta-catenin accumulation through RhoA and ROCK activation. PMID: 27575935
    17. Inhibition of ROCK1 largely blocked Endothelial-to-mesenchymal transition and the increase in endothelial permeability under this high-glucose condition, but overexpression of ROCK1 induced these changes. PMID: 26842599
    18. While the loss of either Rock1 or Rock2 had no negative impact on tumorigenesis in mouse models of non-small cell lung cancer and melanoma, loss of both blocked tumor formation, as no tumors arise in which both Rock1 and Rock2 have been genetically deleted. PMID: 26765561
    19. YQFM ameliorates the OGD-induced brain microvascular endothelial cell barrier disruption associated with the NF-kappaB/p65 and ROCK1/MLC signaling pathways. PMID: 26915982
    20. Extracellular vesicle-encapsulated caspase-3 was largely responsible for the alveolar macrophage activation via the ROCK1 pathway. PMID: 26658190
    21. In experimental autoimmune encephalomyelitis (EAE), ROCK activity was also increased in serum, spleen, brain and spinal cord. Neuron injury with scratch and TNF-alpha stimulation induced the up-regulation of ROCK activity. PMID: 26481340
    22. indicates that the up-regulation of ROCK1 activity in RhoA-deleted macrophages and macrophage phenotype/polarity are dependent on non-apoptotic Caspase-3 PMID: 26875770
    23. Rho-kinase-dependent actin turnover and actomyosin disassembly are necessary for mouse spinal neural tube closure. PMID: 26040287
    24. Results suggest that Rho-associated, coiled-coil containing protein kinase (ROCK) inhibitors fasudil may be an emerging therapeutic option for targeting cancer vasculogenic mimicry (VM). PMID: 25934709
    25. doxorubicin induces caspase activation, necrosis, and actin cytoskeleton alterations largely through ROCK1-dependent and oxidative stress-independent pathways PMID: 26134406
    26. A very rapid reduction in p27-phospho-Ser10 levels at the onset of atherogenesis, which contributes to early plaque build-up through RhoA/ROCK-induced integrin expression, is reported. PMID: 25908026
    27. RhoA and ROCK mediate histamine-induced vascular leakage and anaphylactic shock. PMID: 25857352
    28. ROCK1-dependent changes in hyaluronan may contribute to ROCK1's role in O3-induced AHR, the role of ROCK2 is downstream of inflammation, likely at the level of airway smooth muscle contraction. PMID: 26276827
    29. The results showed that ROCK was essential for early embryo cleavage and blastocyst formation, possibly through a conserved ROCK-LIMK1/2-actin pathway, during mouse embryo development. PMID: 24562870
    30. The role of Rho-kinase in leukocytes during ischemia/reperfusion injury. PMID: 24638037
    31. Proper inner cell mass morphology is dependent on ROCK activity and is crucial for fetal development. PMID: 23946538
    32. Data indicate that transient receptor potential melastatin subtype 8 (TRPM8) activation by menthol attenuated vasoconstriction via RhoA/Rho kinase pathway inhibition in wild-type mice, but the effect was absent in TRPM8(-/-) mice. PMID: 24637663
    33. Adipose ROCK1 isoform plays an inhibtory role for the regulation of insulin sensitivity in diet-induced obesity in vivo. PMID: 24326423
    34. Rho kinase inhibition enhances the survival of newborn neurons in the dentate gyrus. PMID: 23707981
    35. ROCK1(-/-) MEFs showed significant preservation of both focal adhesions and adherens junctions. PMID: 23598717
    36. Data from transgenic/knockout mice suggest loss of Rock1 in AgRP (agouti-related peptide) neurons of arcuate nucleus leads to decrease in energy expenditure/locomotor activity independent of change in food intake and thus leads to obesity/adiposity. PMID: 23885017
    37. the two isoforms of Rho-kinase distribute differentially to accomplish their specific functions PMID: 22986902
    38. Rock1 displays specific control of endothelial cell survival. PMID: 22934846
    39. ROCK1 is involved in destabilizing actin cytoskeleton through regulating MLC2 phosphorylation and peripheral actomyosin contraction. PMID: 23392171
    40. ROCK inhibition diminishes most microglial activation properties, such as the increased cell body size, number of filopodial processes and size of Golgi apparatus, after MPTP insult. PMID: 23139861
    41. data identify ROCK1 as a key regulator of leptin action on energy homeostasis. PMID: 22941110
    42. Rock is thus essential for survival of neural crest cells that form the craniofacial region. PMID: 22629443
    43. ROCK1 functions as a physiologic regulator of p53 under conditions of erythroid stress. PMID: 22889758
    44. Rho-kinase signaling constitures a key feature in streptococcal M1 protein induced acute lung injury. PMID: 22433673
    45. our overall results reveal a model whereby CD1d-mediated Ag presentation is negatively regulated by ROCK1 and ROCK2 via its effects on the actin cytoskeleton. PMID: 22798677
    46. Data suggest that small artery dysfunction in diabetes involves suppression of extracellular glucose dependency of vasoconstriction under hyperglycemic conditions; this dysfunction appear to involve reduced Rho kinase expression. PMID: 22465314
    47. a novel mechanism in which miR-148a positively regulates myogenic differentiation via ROCK1 down-regulation. PMID: 22547064
    48. Regulation of glucose transport by ROCK1 differs from that of ROCK2 and is controlled by actin polymerization. PMID: 22355071
    49. conclude that ROCKDelta1 is a novel fibrotic factor. Activation of TGFbeta1 and NF-kappaB signaling contributes to the Rho kinase-mediated pathological fibrosis PMID: 22278938
    50. Mitochondrial fission triggered by hyperglycemia is mediated by ROCK1 activation in podocytes and endothelial cells. PMID: 22326220

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family
  • 组织特异性：
    Highly expressed in brain, heart, lung, liver, stomach, spleen, kidney, testis, muscle, embryo and placenta.
  • 数据库链接：

    KEGG: mmu:19877

    STRING: 10090.ENSMUSP00000069549

    UniGene: Mm.6710

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