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  4. Recombinant Mouse Low-density lipoprotein receptor-related protein 6 (Lrp6), partial

Recombinant Mouse Low-density lipoprotein receptor-related protein 6 (Lrp6), partial

  • 中文名称：
    小鼠Lrp6重组蛋白
  • 货号：
    CSB-YP013102MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Lrp6重组蛋白
  • 货号：
    CSB-EP013102MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Lrp6重组蛋白
  • 货号：
    CSB-EP013102MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Lrp6重组蛋白
  • 货号：
    CSB-BP013102MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Lrp6重组蛋白
  • 货号：
    CSB-MP013102MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Lrp6
  • Uniprot No.：
    O88572
  • 别名：
    Lrp6; Low-density lipoprotein receptor-related protein 6; LRP-6
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation.
  • 基因功能参考文献：
    1. Cardiac LRP6 deficiency greatly suppressed autophagic degradation and fatty acid utilization, and subsequently leads to lethal dilated cardiomyopathy and cardiac dysfunction through activation of Drp1 signaling. PMID: 29344294
    2. Low LRP6 expression is associated with chronic social defeat stress. PMID: 30201296
    3. In this study, we found that the wnt co-receptor Lrp6 was a potent positive regulator of beta-catenin signaling in TDI-induced asthma models, both in vivo and in vitro. Additionally, for the first time, we demonstrated that RAGE could mediate phosphorylation of Lrp6, suggesting a functional cross talk between RAGE and the canonical wnt/beta-catenin signaling pathway involved in mediating beta-catenin activation. PMID: 29656209
    4. LRP6 acts as a scaffold protein in cardiac gap junction assembly and intracellular protein trafficking. PMID: 27250245
    5. Data show that reduction of alpha chain of nascent polypeptide associated complex (alphaNAC) levels decreased basal expression of Low density lipoprotein receptor-Related Protein 6 (Lrp6) by 30%. PMID: 29413898
    6. GPR37 is required for Wnt/beta-catenin signaling and protects LRP6 from ER-associated degradation via CHIP (carboxyl terminus of Hsc70-interacting protein) and the ATPase VCP PMID: 28341812
    7. VAP1 cleaved recombinant mouse LRP6, producing a 140-kDa fragment. An antibody against a peptide including the LRP6-cleavage site inhibited VAP1-induced VE-cadherin relocation and disruption of cell-cell adhesions and blocked haemorrhage in mice in vivo. PMID: 28425175
    8. we identified Lrp6, the gene encoding the coreceptor to Frizzled in the Wnt pathway, as a potential negative regulator of precursor proliferation. Overexpression and siRNA silencing confirmed the regulatory role of Lrp6 PMID: 26840599
    9. Data (including data from studies in knockout/transgenic mice) suggest Lrp6 is required for suppression of Sost (sclerostin) expression by parathyroid hormone (here, human PTH peptide 1-34); LRP6 regulates osteocytes expression of histone deacetylases. PMID: 25847683
    10. Wt1 expression levels in podocytes regulate Wnt/beta-catenin signaling through modulating the endocytic fate of LRP6, and this indicates a potential target for the therapy of CKD. PMID: 26049137
    11. N-cadherin modulates Lrp6/PTHR1 interaction, restraining the intensity of parathyroid hormone-induced beta-catenin signaling PMID: 25088803
    12. LRP6 restrains vascular smooth muscle lineage noncanonical signals that promote osteochondrogenic differentiation, mediated in part via USF1- and arginine methylation-dependent relays. PMID: 26034040
    13. Urotensin II inhibited the proliferation of cardiac side population cells by JNK/LRP6 signalling during pressure overload. PMID: 24447593
    14. miR-183, though negatively regulated by Gata3, enhances 3T3-L1 preadipocyte differentiation and adipogenesis by targeting LRP6. PMID: 24556500
    15. Phosphorylation of LRP6 was increased after tissue plasminogen activator (tPA) treatment and decreased by tPA siRNA transfection. PMID: 23925701
    16. Results indicate that Lrp6 mediates not only canonical Wnt signaling, but can also modulate non-canonical pathways involving RhoA-dependent mechanisms to impact neurulation, possibly through intracellular complexes with DAAM1. PMID: 23773994
    17. Heterozygous loss-of-function mutation in Lrp6 leads to less beta-catenin signaling within articular cartilage and to increased degenerative joint disease after ligament and meniscus injury. PMID: 23756208
    18. LRP6 in osteoblasts is essential for osteoblastic differentiation during bone remodeling and the anabolic effects of PTH. PMID: 23609180
    19. Low-density lipoprotein receptor-related protein 6 was shown to be a direct target of miR-30e. PMID: 24113179
    20. Hyp/Lrp6 double mutants developed the same reduced bone mineralization,hypophosphatemia & high FGF23 levels as Hyp(-) mice, showing that WNT signaling does not affect the FGF23-induced phosphate wasting. PMID: 23652555
    21. Wnt3a-induced transcriptional responses and induction of alkaline phosphatase activity, an early marker of osteoblast differentiation, require the Wnt co-receptors LRP5 and LRP6 PMID: 23638027
    22. LRP6 activates an endogenous neuroprotective pathway that acts independently of beta-catenin by controlling glycogen-synthase-kinase (GSK)-3beta activity and preventing its deleterious mitochondrial and proinflammatory effects. PMID: 23743975
    23. Arf modulates LRP6 phosphorylation for the transduction of Wnt/beta-catenin signaling. PMID: 22907437
    24. SOX9 regulates low density lipoprotein receptor-related protein 6 (LRP6) and T-cell factor 4 (TCF4) expression and Wnt/beta-catenin activation in breast cancer. PMID: 23306204
    25. DKK-1 acts predominantly by inhibiting PDGF-, TGF-beta-, and connective tissue growth factor-activated MAPK and JNK signaling cascades, acting via LRP-6 with associated WNT ligand. PMID: 23302695
    26. Wnt3a-stimulated LRP6 phosphorylation is dependent upon arginine methylation of G3BP2. PMID: 22357953
    27. The authors report that Dab2, previously described as an inhibitor of Wnt/beta-catenin signalling, selectively recruits LRP6 to the clathrin-dependent endocytic route, thereby sequestering it from caveolin-mediated endocytosis. PMID: 22491013
    28. identifies LRP6 as a nutrient-sensitive regulator of body weight and glucose metabolism and as a potential target for pharmacological interventions in obesity and diabetes. PMID: 22232553
    29. Paternally-expressed gene 1 protein (Peg1) inhibits the maturation and plasma membrane localization of the Wnt co-receptor LRP6 by controlling the glycosylation of LRP6. PMID: 21375506
    30. analysis of the frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6 PMID: 20093360
    31. Wnt5a-/-Lrp6-/- embryos displayed presomitic mesoderm morphogenesis, somitogenesis, and neurogenesis defects, which are much more severe than in either of the single mutants. PMID: 19795512
    32. Lrp6 plays a role in the onset of dopaminergic neuron development in the ventral midbrain as well as a role in midbrain morphogenesis. PMID: 19795519
    33. Lrp6 is essential for cardiac neural crest and outflow tract development. PMID: 19705442
    34. compensatory interactions between Dkk1 and Lrp6 demonstrate the importance of correctly balancing positive and negative regulation of the signaling during mammalian development. PMID: 15115753
    35. role for an LDL receptor-related protein in the regulation of vascular smooth muscle cell proliferation and survival through the evolutionary conserved Wnt signaling cascade. PMID: 15271658
    36. Thalamic development requires normal activity of the LRP6-mediated canonical Wnt signaling pathway. PMID: 15342729
    37. Lrp6 is one of the key genetic components for the pathogenesis of vertebral segmentation defects PMID: 15469977
    38. wingless-related MMTV integration site 7A postaxial hemimelia acts through Lrp6 and the canonical Wnt signaling pathway during dorsal and posterior limb development in the mouse PMID: 15880584
    39. Lrp6 mutation in Crooked tail (Cd) mice provides evidence for a functional connection between Wnt signaling and folate rescue of neural tube defects PMID: 16126904
    40. Together our results show that in addition to serving as a folding chaperone, Mesd can function as a receptor antagonist by inhibiting ligand binding to mature LRP6. PMID: 16263759
    41. Several lines of evidence that the mouse Cristin/R-spondin family proteins function as Fzd8 and LRP6 receptor ligands and induce the canonical Wnt/beta-catenin signaling pathway, leading to TCF-dependent gene activation, are presented. PMID: 16543246
    42. LRP6 mutant mice are hypomorphic for the canonical Wnt signaling pathway and have hypoplasia of the developing neocortex. PMID: 16920270
    43. Enforced expression of full-length human MESD promotes the secretion of soluble minireceptors derived from LRP6 that contain either one or two beta-propeller-EGF domain pairs PMID: 17488095
    44. of receptors and Dvl in LRP6-signalosomes, which in turn triggers LRP6 phosphorylation to promote Axin recruitment and beta-catenin stabilization PMID: 17569865
    45. Results identify a novel feedback mechanism by which Wnt, including Wnt3a, negatively regulates LRP6 at the mRNA level. PMID: 17698587
    46. These findings suggest a critical role of Wnt/Lrp6/beta-catenin signaling in adipogenesis and cell fate decision of mesenchymal stem cells. PMID: 17888405
    47. results indicate that Lrp6-mediated signaling controls postnatal bone mass, at least partly through the regulation of bone resorption PMID: 18505367
    48. DKK1 inhibition of LRP6 is independent of LRP6 internalization and degradation PMID: 18505732
    49. A mechanism whereby LRP6 stabilizes beta-catenin independently of Axin degradation by directly inhibiting GSK3's phosphorylation of beta-catenin, is identified. PMID: 18509060
    50. Insulin-like growth-factor-binding protein-4 physically interacted with a Wnt receptor, Frizzled 8 (Frz8), and a Wnt co-receptor, low-density lipoprotein receptor-related protein 6 (LRP6), and inhibited the binding of Wnt3A to Frz8 and LRP6 PMID: 18528331

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  • 相关疾病：
    Defects in Lrp6 are the cause of Ringelschwanz (rs) phenotype. Rs phenotype is a spontaneous mutation that is characterized by a combination of multiple Wnt-deficient phenotypes including dysmorphologies of the axial skeleton, digits and the neural tube. The establishment of the anteroposterior somite compartments, the epithelialization of nascent somites, and the formation of segment borders are disturbed in (rs) mutants. There is delayed ossification at birth and a low bone mass phenotype in adults. Functional analyzes reveal impaired targeting to the plasma surface due to reduced interaction with MESD leading to inhibited Wnt/beta-catenin signaling.
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Membrane raft.
  • 蛋白家族：
    LDLR family
  • 组织特异性：
    Expressed in early embryo. Broadly expressed throughout the embryonic ectoderm and in nascent mesoderm, and in endoderm emerging from the primitive streak.
  • 数据库链接：

    KEGG: mmu:16974

    STRING: 10090.ENSMUSP00000032322

    UniGene: Mm.321990

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