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  4. Recombinant Mouse Early endosome antigen 1 (Eea1), partial

Recombinant Mouse Early endosome antigen 1 (Eea1), partial

  • 中文名称：
    小鼠Eea1重组蛋白
  • 货号：
    CSB-YP007405MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Eea1重组蛋白
  • 货号：
    CSB-EP007405MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Eea1重组蛋白
  • 货号：
    CSB-EP007405MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Eea1重组蛋白
  • 货号：
    CSB-BP007405MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Eea1重组蛋白
  • 货号：
    CSB-MP007405MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Eea1
  • Uniprot No.：
    Q8BL66
  • 别名：
    Eea1Early endosome antigen 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
  • 基因功能参考文献：
    1. larger autophagic vacuoles that colocalize with early endosome antigen 1 (EEA1) but not with lysosomal-associated membrane protein (LAMP)-1 were much more frequently formed in Rab7(Deltapan) pancreatic acinar cells. PMID: 28588238
    2. Our characterization of the role of EEA1 during homeostatic synaptic plasticity in Mecp2 KO neurons provides potentially novel targets of intervention for improving hippocampal function in Rett Syndrome individuals. PMID: 28621434
    3. In the present study, decreased expression of EEA1 as well as the rearrangement of the actin was observed when Paracoccidioides brasiliensis was internalized in macrophages. PMID: 23566224
    4. Tec vesicles have a membrane proximal location and overlap with the tyrosine kinase Lck, the Tec substrate PLC-gamma1, and the early endosomal antigen 1 marker EEA1 PMID: 15817477
    5. phosphomimetic mutation of Thr-1392 in EEA1 can bypass the requirement for p38alpha in mu opioid receptor endocytosis PMID: 16138080
    6. F. tularensis-containing endosomes associated with early endosome antigen 1 (EEA1) followed by lysosome-associated membrane protein 1 (LAMP-1), with peak coassociation frequencies occurring at 30 and 120 min postinoculation, respectively. PMID: 18426871

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  • 亚细胞定位：
    Cytoplasm. Early endosome membrane; Peripheral membrane protein.
  • 数据库链接：

    KEGG: mmu:216238

    STRING: 10090.ENSMUSP00000061493

    UniGene: Mm.210035

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