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  4. Recombinant Mouse DNA (cytosine-5)-methyltransferase 3B (Dnmt3b), partial

Recombinant Mouse DNA (cytosine-5)-methyltransferase 3B (Dnmt3b), partial

  • 中文名称：
    小鼠Dnmt3b重组蛋白
  • 货号：
    CSB-YP007085MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Dnmt3b重组蛋白
  • 货号：
    CSB-EP007085MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Dnmt3b重组蛋白
  • 货号：
    CSB-EP007085MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Dnmt3b重组蛋白
  • 货号：
    CSB-BP007085MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Dnmt3b重组蛋白
  • 货号：
    CSB-MP007085MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Dnmt3b
  • Uniprot No.：
    O88509
  • 别名：
    Dnmt3bDNA; cytosine-5)-methyltransferase 3B; Dnmt3b; EC 2.1.1.37; DNA methyltransferase MmuIIIB; DNA MTase MmuIIIB; M.MmuIIIB
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells.
  • 基因功能参考文献：
    1. a new paradigm of transcriptional regulation critical for cardiac development and maturation that is controlled by the interaction of REST, DNMT3B and non-CpG methylation. PMID: 27956497
    2. Together, this study described the regulation of Chk2 expression through promoter methylation by Dnmt3b and also presented a novel role of Chk2 during neuronal differentiation, which is independent of its previously known function in DNA damage response. PMID: 28712951
    3. in mouse embryonic stem cells, Dnmt3b-dependent intragenic DNA methylation protects the gene body from spurious RNA polymerase II entry and cryptic transcription initiation PMID: 28225755
    4. Mechanical stimulation regulates osteoblastic genes expression via direct regulation of Dnmt3b. PMID: 28215635
    5. a miR-125b-DNMT3b-p53 signal pathway may exist in the vascular smooth muscle cells proliferation induced by homocysteine. PMID: 27426728
    6. Loss of DNMT3B results in hypomethylation of the miR-196b promoter and increased miR-196b expression, which directly targets the mTORC2 component Rictor. PMID: 26923591
    7. Downregulation of DNMT3B, one of the targets identified using this method, radiosensitizes cancer cells by disturbing multiple DNA damage responses. PMID: 26667181
    8. High levels of Dnmt3b expression prolong leukemia latency. PMID: 26729896
    9. we report that cardiac specific deletion of Dnmt3b, the predominant DNA methyltransferase in adult mouse hearts, leads to an accelerated progression to severe systolic insufficiency and myocardial thinning without a preceding hypertrophic response. PMID: 25784084
    10. Dnmt3b-deficient cells can contribute to the male and female germ-lines in chimeric mice and can produce normal progeny, establishing that Dnmt3b is dispensable for mouse gametogenesis and imprinting. PMID: 20132320
    11. THC in WT mice increased promoter methylation of DNMT3a and DNMT3b in THC-induced myeloid-derived suppressor cells, reducing expression of DNMT3a and DNMT3b. PMID: 25713087
    12. findings reveal how sequence and chromatin cues guide de novo methyltransferase activity to ensure methylome integrity PMID: 25607372
    13. DNMT3b plays an important role in regulating macrophage polarization through epigenetic mechanisms. PMID: 24597547
    14. hyperglycemia-induced oxidative stress stimulates Dnmt3b activity, inhibiting chromatin modifications necessary for Pax3 expression during neurulation, providing a molecular mechanism for defects caused by Pax3 insufficiency in diabetic pregnancy. PMID: 24834974
    15. This sudy demonistrated that Dnmt3b-deficient induced pluripotent stem cells (iPSCs). Single-cell expression analysis revealed that individual Dnmt3b-deficient Purkinje cells expressed increased numbers of Pcdh isoforms. PMID: 24698270
    16. Mice that are homozygous for the Dnmt3b mutant have smaller stature and reduced viability but dis not have cumulative effects across generations. PMID: 23636699
    17. Aldosterone stimulates the dispersal of DNMT3b and recruitment of Tet2 to demethylate the alphaENaC promoter, thereby inducing alphaENaC transcription in kidney collecting duct cells. PMID: 23926181
    18. feeding and hepatic clockwork regulate diurnal variation in Dnmt3b expression. PMID: 22847467
    19. Dnmt3b can act as a haploinsufficient tumor suppressor gene. PMID: 23315164
    20. DNA methyltransferase 3b is dispensable for mouse neural crest development. PMID: 23094090
    21. Dnmt3b is distributed in both the heterochromatin and euchromatin regions. PMID: 15533817
    22. We observe evidence for a context-dependent contribution of Dnmt3b to set and maintain CpG and non-CpG methylation at distinct classes of repetitive elements and selected single copy genes PMID: 22761581
    23. identify Ment (Gm128) as an enhancer of lymphomagenesis that contributes to the tumor suppressor function of Dnmt3b PMID: 22133874
    24. Dnmt3bDelta6 could induce a hypomethylation status especially in in vitro manipulated embryos. PMID: 21666347
    25. In mouse embryonic stem cells, the genes upregulated after deletion of the H3K9 methyltransferase Setdb1 are distinct from those derepressed in mESC deficient in the Dnmt3b, with the exception of a small number of primarily germline-specific genes. PMID: 21624812
    26. DNA methyltransferase 3b methylates genes in a similar way in mouse intestine and human colon cancer PMID: 21490393
    27. Results reveal a compelling new mechanism of RNA-dependent DNA methylation, suggesting that recruitment of DNMT3b by DNA:RNA triplexes may be a common and generally used pathway in epigenetic regulation. PMID: 20952535
    28. Dnmt3b recruitment through E2F6 transcriptional repressor mediates germ-line gene silencing in murine somatic tissues. PMID: 20439742
    29. Lsh is required for establishment of DNA methylation patterns at stem cell genes during differentiation, in part by regulating access of Dnmt3b to its genomic targets. PMID: 19650037
    30. crystal structure: PWWP domain of DNA methyltransferase Dnmt3b defines a new family of DNA-binding folds PMID: 11836534
    31. Dnmt3b is not responsible for maintenance-coupled de novo methylation in mouse cells PMID: 12370304
    32. DNMT3b plays an important role in neoplastic transformation PMID: 12879017
    33. Dnmt3b important for the early phase of neurogenesis. PMID: 15672446
    34. Constitutive or conditional deletion of Dnmt3b, but not Dnmt3a, results in partial loss of DNA methylation throughout the genome, suggesting that Dnmt3b is required for maintaining DNA methylation in mouse embryonic fibroblasts. PMID: 15757890
    35. Dnmt3b plays an essential role at different stages of mouse development, and that Immunodeficiency, Centromeric instability and Facial anomalies missense mutations cause partial loss of function PMID: 16501171
    36. Deletion of Dnmt3b results in suppression of intestinal neoplasms. PMID: 16581773
    37. The Dnmt3b genes showing marginal correlation (-.4 < p(s) < .6) were present in the early stages of differentiation of both GD and RD, which were non-specific to brain development. PMID: 16627687
    38. Transcripts are at their highest levels in type A spermatogonia, decrease dramatically in type B spermatogonia and preleptotene spermatocytes and rise again in leptotene/zygotene spermatocytes. PMID: 16725135
    39. Dnmt3b is specifically expressed in progenitor cells during hematopoiesis, spermatogenesis and neurogenesis, suggesting an important role in the initial steps of progenitor cell differentiation. PMID: 16973295
    40. dnmt3b is coordinately regulated and its expression peaks during the stage of postnatal oocyte development when maternal methylation imprints are established PMID: 17445268
    41. Could cooperate with the orphan receptor COUP-TFI and Dnmt3a to regulate transcription of the vitronectin gene. PMID: 17579209
    42. Both Dnmt3a and Dnmt3b were involved in the methylation of Rasgrf1 DMR and long interspersed repeats IAP and Line1, but only Dnmt3b was required for the methylation of the satellite repeats. PMID: 17616512
    43. Dnmt3b1 enhanced the number of colon tumors in Apc Min/+ mice approximately twofold and increased the average size of colonic microadenomas, whereas Dnmt3a1 had no effect. PMID: 18056424
    44. Data report the characterization of interactions between Dnmt3b and both thymine-DNA glycosylase (Tdg) and methyl-CpG binding domain protein 4. PMID: 18452947
    45. Insufficient methylation of the DNMT3L promoter during embryonic development due to deficiency in DNMT3B might be implicated in the pathogenesis of the ICF syndrome (characterized by immunodeficiency, centromere instability and facial anomalies). PMID: 18544626

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  • 亚细胞定位：
    Nucleus. Note=Accumulates in the major satellite repeats at pericentric heterochromatin.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, C5-methyltransferase family
  • 数据库链接：

    KEGG: mmu:13436

    STRING: 10090.ENSMUSP00000051830

    UniGene: Mm.491804

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