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  4. Recombinant Human NEDD4-like E3 ubiquitin-protein ligase WWP1 (WWP1), partial

Recombinant Human NEDD4-like E3 ubiquitin-protein ligase WWP1 (WWP1), partial

  • 中文名称：
    人WWP1重组蛋白
  • 货号：
    CSB-YP872425HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人WWP1重组蛋白
  • 货号：
    CSB-EP872425HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人WWP1重组蛋白
  • 货号：
    CSB-EP872425HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人WWP1重组蛋白
  • 货号：
    CSB-BP872425HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人WWP1重组蛋白
  • 货号：
    CSB-MP872425HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    WWP1
  • Uniprot No.：
    Q9H0M0
  • 别名：
    WWP1; NEDD4-like E3 ubiquitin-protein ligase WWP1; EC 2.3.2.26; Atrophin-1-interacting protein 5; AIP5; HECT-type E3 ubiquitin transferase WWP1; TGIF-interacting ubiquitin ligase 1; Tiul1; WW domain-containing protein 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

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WWP1 antibodies

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF.
  • 基因功能参考文献：
    1. Study identified beta-dystroglycan as a substrate of WWP1 and found that the muscular dystrophy-causing mutation of WWP1 renders the enzyme hyperactive by relieving autoinhibition. PMID: 29635000
    2. Results show that WWP1 is frequently upregulated in gastric cancer (GC) tissues and cells, and that its 3'-UTR is a putative target of miR-584 which suppresses its protein expression by mRNA degradation. PMID: 28431583
    3. study describes a physical and functional interaction between Ebola virus VP40 (eVP40) and WWP1, a host E3 ubiquitin ligase that ubiquitinates VP40 and regulates virus-like particles egress. PMID: 28768865
    4. Knock-down of WWP1 abrogates DNA damage-induced down-regulation of DeltaNp63alphaand partially rescues cell apoptosis PMID: 28426804
    5. Describe an autoinhibitory mechanism for WWP1 ubiquitin ligase involving a linker-HECT domain interaction. This intramolecular interaction traps the HECT enzyme in its inactive state and can be relieved by linker phosphorylation. PMID: 28475870
    6. results demonstrate that WWP1 catalyzes the formation of Ub chains through a sequential addition mechanism, in which Ub monomers are transferred in a successive fashion to the substrate, and that ubiquitination by WWP1 requires the presence of a low-affinity, noncovalent Ub-binding site within the HECT domain. PMID: 28461335
    7. Study shows that WWP1 was upregulated in prostate cancer (PCa) clinical specimens and contributed to cancer cell invasion, indicating that this target of Mir-452 functioned as an oncogene. PMID: 27070713
    8. Overexpression of WWP1 promotes tumorigenesis in patients with hepatocellular carcinoma. PMID: 26506518
    9. PTPN14, a Pez mammalian homolog, is degraded by overexpressed Su(dx) or Su(dx) homologue WWP1 in mammalian cells. PMID: 25814387
    10. miR-21 overexpression or WWP1 knockdown in endothelial progenitor cells significantly activates the TGFbeta signaling pathway and inhibits cell proliferation. PMID: 25755729
    11. The cancer-driven alteration of WWP1 culminates in excessive TbetaRI degradation and attenuated TGFbeta1 cytostatic signaling, a consequence that could conceivably confer tumorigenic properties to WWP1. PMID: 26152726
    12. Results suggest that elevated transcription and expression levels of ubiquitin ligase E3s WWP1, Smurf1 and Smurf2 genes may be the mechanisms of occurrence, development and metastasis of prostate cancer. PMID: 25051198
    13. Most notably, WWP1 downregulation both inactivated PTEN-Akt signaling pathway in MKN-45 and AGS cells. our findings suggest that WWP1 acts as an oncogenic factor and should be considered as a novel interfering molecular target for gastric carcinoma PMID: 25293520
    14. Data show that WWP1, which specifically ubiquitinates and degrades DeltaNp73 heterodimerizes with another E3 ligase, WWP2. PMID: 25071155
    15. Knocking down WWP1 promoted cleaved caspase3 protein and p53 expression in hepatocellular carcinoma cells, and caspase3 inhibition could prevent cell apoptosis induced by the knockdown of WWP1. PMID: 24792179
    16. results reveal that WWP1 might play an oncogenic role in oral cancer cells. PMID: 23849376
    17. LATS1 is critical in mediating WWP1-induced increased cell proliferation in breast cancer cells. PMID: 23573293
    18. WWP1 down-regulates AMPKalpha2 under high glucose culture conditions via the ubiquitin-proteasome pathway PMID: 23293026
    19. WWP1 negatively regulates cell migration to CXCL12 by limiting CXCR4 degradation to promote breast cancer metastasis to bone. PMID: 22266093
    20. WWP1 may function as the E3 ligase for several PY motif-containing proteins, such as Smad2, KLF5, p63, ErbB4/HER4, RUNX2, JunB, RNF11, SPG20, and Gag, as well as several non-PY motif containing proteins, such as TbetaR1, Smad4, KLF2, and EPS15. Review. PMID: 22051607
    21. WWP1 depletion-induced apoptosis was rescued by the overexpression of the wild-type WWP1 but not the E3 ligase inactive WWP1-C890A mutant in MCF7 cells. PMID: 21480222
    22. TAZ promotes breast cell growth partially through protecting KLF5 from WWP1-mediated degradation and enhancing KLF5's activities. PMID: 22045023
    23. WWP1 mutations are not a common cause of human dystroglycanopathy. PMID: 21996799
    24. WWP1 markedly inhibited the replicative senescence induced by p27(Kip1) by promoting p27(Kip1) degradation. PMID: 21795702
    25. The authors show that WWP1 changes the ubiquitination status of ARRDC1, suggesting that the arrestins may provide a platform for ubiquitination in PPXY-dependent budding. PMID: 21191027
    26. WWP1 regulates DeltaNp63 transcriptional activity, acting thus as a potential regulator of the proliferation and survival of epithelial-derived cells. PMID: 20951678
    27. First demonstration of ubiquitin-protein ligase WWP1 from human lung cDNA library recruited by penton base proteins of human adenovirus serotypes Ad2 and Ad3 in vitro and in vivo. PMID: 12450395
    28. Data show that the crystal structure of the HECT domain of the human ubiquitin ligase WWP1/AIP5 maintains a two-lobed structure like the HECT domain of the human ubiquitin ligase E6AP. PMID: 12535537
    29. WWP1 negatively regulates TGF-beta signaling in cooperation with Smad7. PMID: 15221015
    30. KLF5 is a target of the E3 ubiquitin ligase WWP1 for proteolysis in epithelial cells PMID: 16223724
    31. Full-length expressed WWP1 could interact in vitro with the cytoplasmic domain of human Notch1, which also regulate the nuclear localization of WWP1. PMID: 16785210
    32. these findings identify the first instance of a ubiquitin ligase that causes stabilization of p53 while inactivating its transcriptional activities. PMID: 16924229
    33. WWP1 overexpression is a common mechanism involved in the inactivation of TGFbeta function in human cancer. PMID: 17016436
    34. genomic aberrations of WWP1 may contribute to the pathogenesis of breast cancer PMID: 17330240
    35. the interaction between Gag and WWP1 is required for functions other than Gag ubiquitination PMID: 17609263
    36. WWP1 may promote cell proliferation and survival partially through suppressing RNF11-mediated ErbB2 and EGFR downregulation in human cancer cells. PMID: 18724389
    37. WWP1 may have a context-dependent role in regulating cell survival through targeting different p63 proteins for degradation. PMID: 18806757
    38. analysis of the interaction between ubiquitin ligase WWP1 and Nogo-A PMID: 19035836
    39. WWP1 ubiquitinated and caused the degradation of HER4 but not of EGFR, HER2, or HER3. PMID: 19047365
    40. Overexpression of WWP1 is associated with the estrogen receptor and insulin-like growth factor receptor 1 in breast carcinoma PMID: 19267401
    41. Experiments suggest functions for WWP1 and SPG20 in the regulation of lipid droplet turnover and potential pathological mechanisms in Troyer syndrome. PMID: 19307600
    42. WWP1 and its family members suppress the ErbB4 expression and function in breast cancer PMID: 19561640
    43. SPG20 interacts with AIP4 and AIP5. PMID: 19580544
    44. the interaction of nonenveloped viruses with ubiquitin-protein ligases of host cells. PMID: 12450395

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  • 亚细胞定位：
    Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus.
  • 组织特异性：
    Detected in heart, placenta, pancreas, kidney, liver, skeletal muscle, bone marrow, fetal brain, and at much lower levels in adult brain and lung. Isoform 1 and isoform 5 predominate in all tissues tested, except in testis and bone marrow, where isoform 5
  • 数据库链接：

    HGNC: 17004

    OMIM: 602307

    KEGG: hsa:11059

    STRING: 9606.ENSP00000265428

    UniGene: Hs.655189

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