Recombinant Human Interferon kappa (IFNK)

^{In Stock}

中文名称：

人IFNK重组蛋白
货号：

CSB-EP889172HU
规格：

￥1836
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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纯度：

Greater than 85% as determined by SDS-PAGE.
基因名：

IFNK
Uniprot No.：

Q9P0W0
种属：

Homo sapiens (Human)
蛋白长度：

Full Length of Mature Protein
来源：

E.coli
分子量：

35.1 kDa
表达区域：

28-207aa
氨基酸序列

LDCNLLNVHLRRVTWQNLRHLSSMSNSFPVECLRENIAFELPQEFLQYTQPMKRDIKKAFYEMSLQAFNIFSQHTFKYWKERHLKQIQIGLDQQAEYLNQCLEEDKNENEDMKEMKENEMKPSEARVPQLSSLELRRYFHRIDNFLKEKKYSDCAWEIVRVEIRRCLYYFYKFTALFRRK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：

N-terminal 6xHis-SUMO-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

3-7 business days
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
产品描述：

This recombinant Human IFNK protein is typically achieved by the manipulation of IFNK gene expression in E.coli cells so that it expresses large amounts of a recombinant IFNK gene. In order to get enough amount of the IFNK protein, strain selection, codon optimization, fusion systems, co-expression, mutagenesis, and isotope labeling techniques are used. Finally, the IFNK protein is isolated from the samples such as cell lysates or medium. Protein refolding, cleavage of fusion moieties and chromatography techniques are involved in the protein purification process.
IFNK is a multifunctional type I IFN that elicits autoimmunity when transgenically expressed in the β cells of the pancreatic islets in mice. IFNK is induced after viral infection or treatment of cells with double-stranded RNA (dsRNA). IFNK signaling stimulates a collection of genes, including IRF1, STAT1, MXA, PKR, and OAS1, which are common to signaling via the type I interferon receptor, and modulates the release of cytokines from monocytes. Different from other members of the type I IFN family, IFNK is constitutively and highly expressed in human keratinocytes. IFNK plays an important role in keratinocyte host defense against herpes simplex virus-1 (HSV-1).
Datasheet & COA：

Please contact us to get it.

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功能：

May play a role in the regulation of immune cell function. Cytokine that imparts cellular protection against viral infection in a species-specific manner. Activates the interferon-stimulated response element signaling pathway. It is able to directly modulate cytokine release from monocytes and dendritic cells. Binds heparin.
基因功能参考文献：
1. investigation of common variable immunodeficiency (CVID) from 2 German families; report on the occurrence of a common and one novel truncating IFNK mutation in cases with CVID; the frequency distribution of c.30_31insTGTT in cases and controls as well as the observed segregation patterns in CVID families exclude IFNK mutations as major risk factor in CVID PMID: 28324805
2. These results suggest that high-risk human papillomavirus 31 target interferon kappa to prevent Sp100 expression and identify Sp100 as an interferon-stimulated gene with anti-human papillomavirus activity. PMID: 26491169
3. This study demonstrates that E2 proteins of high risk human papillomavirus reduce STING and IFN-kappa transcription. PMID: 24614210
4. The viral E6 and E7 oncogenes are sufficient for interferon-kappa repression, with E6 being mainly responsible. PMID: 21849431
5. IFN-kappa is down-regulated in cervical keratinocytes harboring HPV, which may be a contributing factor in the progression of a cervical lesion PMID: 20479716
6. Studied IFNK single nucleotide polymorphisms in 3982 Systemic lupus erythematosus cases and 4275 controls. PMID: 20706608
7. IFN-kappa is able to directly modulate cytokine release from monocytes and dendritic cells, inhibit inducible IL-12 release from monocytes, and bind strongly to heparin. PMID: 12391192
8. This is the first report showing an epigenetic silencing of type I IFN after HPV16 oncogene expression PMID: 19887612
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亚细胞定位：

Secreted.
蛋白家族：

Alpha/beta interferon family
组织特异性：

Expressed in keratinocytes, monocytes and in resting dendritic cells.
数据库链接：

HGNC: 21714

OMIM: 615326

KEGG: hsa:56832

STRING: 9606.ENSP00000276943

UniGene: Hs.591083

Recombinant Human Interferon kappa (IFNK)

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