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  4. Human Interferon kappa(IFNK) ELISA kit

Human Interferon kappa(IFNK) ELISA kit

说明书下载
  • 中文名称：
    人干扰素κ(IFNK)酶联免疫试剂盒
  • 货号：
    CSB-EL011053HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人干扰素κ(IFNK)酶联免疫试剂盒（CSB-EL011053HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNK含量。IFNK即干扰素κ，是I型干扰素家族成员。它具有抗病毒、抗肿瘤和免疫调节等潜在作用。其研究机制方面，能与细胞表面受体结合，激活相关信号通路，诱导多种抗病毒蛋白产生，发挥免疫调节活性，抵御病原体入侵与肿瘤发展。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于病毒感染机制、自身免疫疾病模型构建、皮肤屏障功能研究等科研领域，还特别适用于免疫微环境分析、细胞因子网络研究等基础科研场景，能为探索IFNK在炎症性疾病、肿瘤免疫调控及组织特异性免疫应答中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    IFN kappa ELISA Kit; IFN-kappa ELISA Kit; Ifnk ELISA Kit; IFNK_HUMAN ELISA Kit; Interferon kappa ELISA Kit
  • 缩写：
    IFNK
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IFNK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 93    
    Range % 89-97    
    1:2 Average % 85    
    Range % 80-90    
    1:4 Average % 90    
    Range % 85-94    
    1:8 Average % 98    
    Range % 94-103    
  • 回收率：
    The recovery of human IFNK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 101 97-105    
    EDTA plasma (n=4) 92 88-96    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.694 2.758 2.726 2.622    
    500 2.309 2.182 2.246 2.142    
    250 1.515 1.601 1.558 1.454    
    125 0.954 1.004 0.979 0.875    
    62.5 0.521 0.560 0.541 0.437    
    31.25 0.287 0.293 0.290 0.186    
    15.6 0.193 0.205 0.199 0.095    
    0 0.100 0.107 0.104 　    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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IFNK Proteins

靶点详情

  • 功能：
    May play a role in the regulation of immune cell function. Cytokine that imparts cellular protection against viral infection in a species-specific manner. Activates the interferon-stimulated response element signaling pathway. It is able to directly modulate cytokine release from monocytes and dendritic cells. Binds heparin.
  • 基因功能参考文献：
    1. investigation of common variable immunodeficiency (CVID) from 2 German families; report on the occurrence of a common and one novel truncating IFNK mutation in cases with CVID; the frequency distribution of c.30_31insTGTT in cases and controls as well as the observed segregation patterns in CVID families exclude IFNK mutations as major risk factor in CVID PMID: 28324805
    2. These results suggest that high-risk human papillomavirus 31 target interferon kappa to prevent Sp100 expression and identify Sp100 as an interferon-stimulated gene with anti-human papillomavirus activity. PMID: 26491169
    3. This study demonstrates that E2 proteins of high risk human papillomavirus reduce STING and IFN-kappa transcription. PMID: 24614210
    4. The viral E6 and E7 oncogenes are sufficient for interferon-kappa repression, with E6 being mainly responsible. PMID: 21849431
    5. IFN-kappa is down-regulated in cervical keratinocytes harboring HPV, which may be a contributing factor in the progression of a cervical lesion PMID: 20479716
    6. Studied IFNK single nucleotide polymorphisms in 3982 Systemic lupus erythematosus cases and 4275 controls. PMID: 20706608
    7. IFN-kappa is able to directly modulate cytokine release from monocytes and dendritic cells, inhibit inducible IL-12 release from monocytes, and bind strongly to heparin. PMID: 12391192
    8. This is the first report showing an epigenetic silencing of type I IFN after HPV16 oncogene expression PMID: 19887612

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 组织特异性：
    Expressed in keratinocytes, monocytes and in resting dendritic cells.
  • 数据库链接：

    HGNC: 21714

    OMIM: 615326

    KEGG: hsa:56832

    STRING: 9606.ENSP00000276943

    UniGene: Hs.591083

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |