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  4. Recombinant Human Gem-associated protein 5 (GEMIN5), partial

Recombinant Human Gem-associated protein 5 (GEMIN5), partial

  • 中文名称：
    人GEMIN5重组蛋白
  • 货号：
    CSB-YP844733HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人GEMIN5重组蛋白
  • 货号：
    CSB-EP844733HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人GEMIN5重组蛋白
  • 货号：
    CSB-EP844733HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人GEMIN5重组蛋白
  • 货号：
    CSB-BP844733HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人GEMIN5重组蛋白
  • 货号：
    CSB-MP844733HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GEMIN5
  • Uniprot No.：
    Q8TEQ6
  • 别名：
    GEMIN5Gem-associated protein 5; Gemin5
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Within the SMN complex, GEMIN5 recognizes and delivers the small nuclear RNAs (snRNAs) to the SMN complex. Binds to the 7-methylguanosine cap of RNA molecules. Binds to the 3'-UTR of SMN1 mRNA and regulates its translation; does not affect mRNA stability. May play a role in the regulation of protein synthesis via its interaction with ribosomes.
  • 基因功能参考文献：
    1. the WD40 domain of Gemin5 is both necessary and sufficient for binding the Sm site of pre-snRNAs PMID: 27881600
    2. provide mechanistic understandings of Gemin5's snRNA-binding specificity as well as valuable insights into the molecular mechanism of RNA binding by WD40 repeat proteins in general. PMID: 27881601
    3. These results reveal the ribosome-binding capacity of the N-ter moiety, enabling Gemin5 to control global protein synthesis. PMID: 27507887
    4. Gemin5 is dispensable for snRNA identification and snRNP assembly. PMID: 26069323
    5. This work both reveals a new autoregulatory pathway governing SMN expression, and identifies a new mechanism through which SMN can modulate specific mRNA expression via Gemin5. PMID: 25911097
    6. The C-terminal region of Gemin5 bears two non-canonical bipartite RNA-binding sites. PMID: 24598255
    7. The Gemin5's function in delivering pre-snRNAs as substrates for Sm core assembly and processing. PMID: 20513430
    8. gemin5 has a role as a novel cap-binding protein and WD repeat domains are involved in m(7)G recognition PMID: 19750007
    9. we report the identification of an additional component of the SMN complex, a novel WD repeat protein termed Gemin5. Gemin5 binds SMN directly and is a component of the SMN complex PMID: 11714716
    10. Gemin5 is the snRNA binding protein of the SMN complex, binding directly and specifically to the unique features of snRNAs and is the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis PMID: 16857593
    11. Gemin5 functions as a scaffold protein for the ASK1-JNK1 signaling module and thereby potentiates ASK1-mediated signaling events. PMID: 17541429
    12. Absence of GEMIN5 from SMN complexes in nuclear Cajal bodies is demonstrated. PMID: 17640370
    13. Gemin5-containing subunits bind small nuclear RNA independently of the SMN complex and without a requirement for exogenous ATP PMID: 17640873
    14. The data provide the first demonstration that alterations in the expression of Gemin5, a spliceosome protein, can effect both specific splicing events and tumor cell motility. PMID: 18245461
    15. The entire WD repeat domain, comprising 13 WD motifs, is both necessary and sufficient for sequence-specific, high-affinity binding of Gemin5 to its RNA targets. PMID: 19377484

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  • 亚细胞定位：
    Nucleus, nucleoplasm. Nucleus, gem. Cytoplasm.
  • 蛋白家族：
    WD repeat gemin-5 family
  • 数据库链接：

    HGNC: 20043

    OMIM: 607005

    KEGG: hsa:25929

    STRING: 9606.ENSP00000285873

    UniGene: Hs.483921

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