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  4. Recombinant Human Arachidonate 12-lipoxygenase, 12S-type (ALOX12)

Recombinant Human Arachidonate 12-lipoxygenase, 12S-type (ALOX12)

  • 中文名称：
    Recombinant Human Arachidonate 12-lipoxygenase, 12S-type(ALOX12)
  • 货号：
    CSB-YP001617HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human Arachidonate 12-lipoxygenase, 12S-type(ALOX12)
  • 货号：
    CSB-EP001617HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human Arachidonate 12-lipoxygenase, 12S-type(ALOX12)
  • 货号：
    CSB-BP001617HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human Arachidonate 12-lipoxygenase, 12S-type(ALOX12)
  • 货号：
    CSB-MP001617HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ALOX12
  • Uniprot No.：
    P18054
  • 别名：
    12 LOX; 12(S) lipoxygenase ; 12-lipoxygenase; 12LO; 12S LOX; 12S-lipoxygenase; 12S-LOX; 12S-type; Alox12; Arachidonate 12 lipoxygenase; Arachidonate 12 lipoxygenase, 12S type; Arachidonate 12-lipoxygenase; Arachidonate 12-oxidoreductase; Lipoxin synthase 12-LO; LOG12; LOX12_HUMAN; P-12LO; Platelet 12-LOX; Platelet type lipoxygenase 12 ; platelet-type 12-lipoxygenase; Platelet-type lipoxygenase 12
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-663
  • 氨基酸序列
    MGRYRIRVAT GAWLFSGSYN RVQLWLVGTR GEAELELQLR PARGEEEEFD HDVAEDLGLL QFVRLRKHHW LVDDAWFCDR ITVQGPGACA EVAFPCYRWV QGEDILSLPE GTARLPGDNA LDMFQKHREK ELKDRQQIYC WATWKEGLPL TIAADRKDDL PPNMRFHEEK RLDFEWTLKA GALEMALKRV YTLLSSWNCL EDFDQIFWGQ KSALAEKVRQ CWQDDELFSY QFLNGANPML LRRSTSLPSR LVLPSGMEEL QAQLEKELQN GSLFEADFIL LDGIPANVIR GEKQYLAAPL VMLKMEPNGK LQPMVIQIQP PNPSSPTPTL FLPSDPPLAW LLAKSWVRNS DFQLHEIQYH LLNTHLVAEV IAVATMRCLP GLHPIFKFLI PHIRYTMEIN TRARTQLISD GGIFDKAVST GGGGHVQLLR RAAAQLTYCS LCPPDDLADR GLLGLPGALY AHDALRLWEI IARYVEGIVH LFYQRDDIVK GDPELQAWCR EITEVGLCQA QDRGFPVSFQ SQSQLCHFLT MCVFTCTAQH AAINQGQLDW YAWVPNAPCT MRMPPPTTKE DVTMATVMGS LPDVRQACLQ MAISWHLSRR QPDMVPLGHH KEKYFSGPKP KAVLNQFRTD LEKLEKEITA RNEQLDWPYE YLKPSCIENS VTI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

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靶点详情

  • 功能：
    Catalyzes the regio and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species. Mainly converts arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to the specific bioactive lipid (12S)-hydroperoxyeicosatetraenoate/(12S)-HPETE. Through the production of bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It can also catalyze the epoxidation of double bonds of polyunsaturated fatty acids such as (14S)-hydroperoxy-docosahexaenoate/(14S)-HPDHA resulting in the formation of (13S,14S)-epoxy-DHA. Furthermore, it may participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively downregulate the immune response and have anti-aggregation properties with platelets. An additional function involves a multistep process by which it transforms leukotriene A4/LTA4 into the bioactive lipids lipoxin A4/LXA4 and lipoxin B4/LXB4, both are vasoactive and LXA4 may regulate neutrophil function via occupancy of specific recognition sites. Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to (13S)-hydroperoxyoctadecadienoate/ (13S-HPODE). Due to its role in regulating both the expression of the vascular endothelial growth factor (VEGF, an angiogenic factor involved in the survival and metastasis of solid tumors) and the expression of integrin beta-1 (known to affect tumor cell migration and proliferation), it can be regarded as protumorigenic. Important for cell survival, as it may play a role not only in proliferation but also in the prevention of apoptosis in vascular smooth muscle cells.
  • 基因功能参考文献：
    1. ALOX15 orthologs, commonly known as 12/15-lipoxygenases, were suggested to exhibit mainly arachidonic acid 12-lipoxygenating specificity in mammals ranked in evolution lower than gibbons and 15-lipoxygenating specificity in the higher ranking primates [Review]. PMID: 30237084
    2. ALOX12 rs14309 GG genotype expression was found to be significantly associated with Cardiovascular events in patients with Diabetic Nephropathy. PMID: 29196930
    3. Data suggest that ALOX12 is involved in oxidative stress and endoplasmic reticulum stress in liver and other tissues leading to non-alcoholic fatty liver disease. [REVIEW] PMID: 28886991
    4. 12(S)-HETrE, a 12-lipoxygenase oxylipin of dihomo-gamma-linolenic acid, inhibits thrombosis via Galphas signaling in platelets. PMID: 27470510
    5. Results identified a novel ALOX12 locus (indicated by two SNPs in perfect linkage disequilibrium: rs1042357 and rs10852889) that moderated the association between PTSD and reduced thickness of the right prefrontal cortex. PMID: 26372769
    6. The regulation of important oxylipin metabolic genes in peripheral blood mononuclear cells varied with the extent of change in arachidonic acid concentrations in the case of PTGS1 and ALOX12 regulation. PMID: 26672987
    7. ITGB4 stimulation leads to recruitment of 12-LOX from the cytosol to the membrane. PMID: 26037302
    8. In the highest tertile with ALOX12. PMID: 25339205
    9. the amount of LPL expressed in muscle and heart governed both the binding of chylomicron particles and the assimilation of chylomicron lipids in the tissue. PMID: 25598081
    10. Results suggest that Alox12 protein polymorphisms did not discriminate for psoriasis severity. PMID: 24975552
    11. Results indicate that secreted phospholipase A2 IIA (sPLA2-IIA) as an enzyme working in concert with platelet microparticles (MPs) 12-lipoxygenase (12-LO) to promote internalization. PMID: 26106157
    12. IGF-1 reduces lipid oxidation and foam cell formation via downregulation of 12/15-LOX to prevent atherosclerosis. PMID: 25549319
    13. The 12/15-lipoxygenase as an emerging therapeutic target for Alzheimer's disease PMID: 25708815
    14. The costaining of pancreatic polypeptide and vimentin suggests that 12-LO participates in the process leading to beta-cell dedifferentiation in the islet. PMID: 25532042
    15. The ALOX12 levels were relatively decreased in nasal polyp tissue. PMID: 22991985
    16. Inhibition of 12-LOX activity significantly sensitizes prostate cancer cells to radiation. PMID: 25260086
    17. Our goal was to investigate if 12-LOX and/or PAI-1 in patient's plasma could be used to predict outcome of the disease. PMID: 24783193
    18. There was no statistically significant difference in expression of ALOX12 mRNA in cholesteatoma vs. control tissue. PMID: 23832258
    19. This study demonstrated the critical role ALOX12 plays in T. gondii infection in humans, using a monocytic cell line with an ALOX12 knockdown. PMID: 24686056
    20. 12-LOX 261Arg > Gln polymorphism is associated with rectal cancer. PMID: 23715757
    21. PAR4 and GPVI-mediated platelet reactivity involves 12-lipoxygenase PMID: 23784669
    22. The results of our study failed to confirm whether the selected variants in ALOX12 gene within the LT metabolism pathway contribute to platelet reactivity in a diabetic population treated with ASA. PMID: 23828562
    23. Our results suggest that LOX metabolites such as 12-HETE are critical in prostate cancer progression and that the LOX pathway may be a target for treating and preventing prostate cancer. PMID: 22895552
    24. the authors have identified a new pathway by which overexpression of 12-LOX in prostate cancer cells leads to augmented production of MMP9 via activation of PI3K/Akt/NF-kappaB signaling. PMID: 23526143
    25. The LOX isozymes 12 and 15 contribute differentially to striatal vulnerability in response to neurotoxicant challenge. PMID: 23079635
    26. Findings suggest 12/15-LOX expressed in non-epithelial cells such as macrophages and fibroblasts leads to bronchial epithelial injury. PMID: 23528921
    27. Pharmacological inhibition and antisense knockdown of 12LO results in apoptosis of human vascular smooth muscle cells. PMID: 23578768
    28. increased 15-LOX-1 and decreased 5- and 12-LOX levels at the onset of kidney cancer reversing with the progressing stage of the disease or the grade of tumor PMID: 22825379
    29. Non-synonymous polymorphism (Gln261Arg) of 12-lipoxygenase is associated with colorectal and thyroid cancers. PMID: 22864639
    30. results show an increase of ALOX15B during the differentiation of monocytes to macrophages, while the expression of ALOX12 and ALOX15 remains on the same low level. PMID: 22980500
    31. results suggest that genetic variations in arachidonate 12-lipoxygenase (ALOX12) are associated with both the onset and cessation of menstruation in Chinese women living in Shanghai PMID: 22668814
    32. DNA methylation analysis of ALOX12 and GSTM1 in acute myeloid leukaemia identifies prognostically significant groups. PMID: 22924777
    33. The results suggest that the genetic polymorphisms in both human ALOX12 and ALOX15 may contribute to variations in the peak BMD of Chinese women. PMID: 22089472
    34. ALOX12 may have a role in growth of breast cancer, and its inhibition may be a strategy for inhibiting tumor growth PMID: 22384268
    35. DNA hypomethylation associated with persistent wheezing in childhood PMID: 22323304
    36. 12(S)-LOX expression in inflammatory areas of colorectal tumours has the capacity to induce an invasive phenotype in colorectal cancer cells and could be targeted for therapy. PMID: 22237009
    37. Overexpression of 12/15-lipoxygenase leads to increased levels of beta-secretase-1 mRNA and protein, a significant elevation in amyloid beta levels and deposition, and a worsening of memory deficits in Alzheimer's disease transgenic mice. PMID: 22275252
    38. genetic variants in ALOX12 may influence BMD and fracture risk. PMID: 21104233
    39. This study for the first time had co-related the quantity of serum LOX-12 with breast cancer and also with the effect of chemotherapy. PMID: 21945939
    40. 12-LOX may serve as a unique marker and therapeutic target for prostate cancer stem cells PMID: 21225230
    41. the rs2073438 polymorphism of ALOX12 contributes to the variation of obesity phenotypes in young Chinese men PMID: 20697415
    42. Data suggest that the development of combinatory therapies that profit from the ACSL4, lipooxygenase and COX-2 synergistic action may allow for lower medication doses and avoidance of side effects. PMID: 21085606
    43. Protease-activated receptor signaling in platelets activates cytosolic phospholipase A2alpha differently for cyclooxygenase-1 and 12-lipoxygenase catalysis. PMID: 21127289
    44. Genetic absence of this enzyme results in a significant reduction in amyloid-beta (Abeta) production and deposition and an improvement of cognitive deficits. PMID: 20570249
    45. the 12 and 15 ALOX expression, localization and downstream cytokine expression in subcutaneous and omental adipose tissue in human obesity were shown. PMID: 21094135
    46. Thrombin-activated human platelets acutely generate oxidized docosahexaenoic-acid-containing phospholipids via 12-lipoxygenase PMID: 20653566
    47. The mRNA levels of ALOX12 implicated pathways differed significantly in gliomas according to the histological type. PMID: 20382140
    48. we suggest that single nucleotide polymorphisms of the ALOX12 gene might be associated with schizophrenia and negative symptoms in this Korean population PMID: 20626912
    49. Two single-nucleotide polymorphisms, rs9904779 and rs434473 (encodes a replacement of asparagine by serine), of arachidonate 12-lipoxygenase were significantly associated with age at natural menopause PMID: 20061896
    50. ALOX12 is a direct transcriptional target of RUNX1. PMID: 20181616

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  • 相关疾病：
    Esophageal cancer (ESCR); Colorectal cancer (CRC)
  • 亚细胞定位：
    Cytoplasm, cytosol. Membrane. Note=Membrane association is stimulated by EGF.
  • 蛋白家族：
    Lipoxygenase family
  • 组织特异性：
    Expressed in vascular smooth muscle cells.
  • 数据库链接：

    HGNC: 429

    OMIM: 114500

    KEGG: hsa:239

    STRING: 9606.ENSP00000251535

    UniGene: Hs.654431

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