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  4. Recombinant Enterobacteria phage T4 Pre-baseplate central spike protein Gp5 (5), partial

Recombinant Enterobacteria phage T4 Pre-baseplate central spike protein Gp5 (5), partial

  • 中文名称：
    Recombinant Enterobacteria phage T4 Pre-baseplate central spike protein Gp5(5),partial
  • 货号：
    CSB-YP321413EDZ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage T4 Pre-baseplate central spike protein Gp5(5),partial
  • 货号：
    CSB-EP321413EDZ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage T4 Pre-baseplate central spike protein Gp5(5),partial
  • 货号：
    CSB-EP321413EDZ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage T4 Pre-baseplate central spike protein Gp5(5),partial
  • 货号：
    CSB-BP321413EDZ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage T4 Pre-baseplate central spike protein Gp5(5),partial
  • 货号：
    CSB-MP321413EDZ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    5
  • Uniprot No.：
    P16009
  • 别名：
    5Baseplate central spike complex protein gp5; Peptidoglycan hydrolase gp5; EC 3.2.1.17; Protein Gp5) [Cleaved into: Gp5*; Gp5C]
  • 种属：
    Enterobacteria phage T4 (Bacteriophage T4)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Baseplate central spike complex-associated lysozyme that is essential for the localized hydrolysis of bacterial cell wall, so that the tail tube, through which the phage DNA is ejected, can penetrate to the host inner membrane. The tail lysozyme complex at the tip of the tail tube penetrates through the outer membrane into the periplasm and during that process, gp5* dissociates from gp5C and activated. Due to the lower pH in the periplasm, gp5* would dissociate from gp27 which probably still binds to the tip of the tube. This way, lysozyme domain is released and locally digests the peptidoglycan layer to make a hole to let the tube penetrate to the inner membrane. Involved in the tail assembly.
  • 基因功能参考文献：
    1. Data suggest that the buried binding pocket of lysozyme can be manipulated (here using double mutant L99A/M102Q) to change observed affinity and thus free energy of binding as ligands bind this site. PMID: 25212393
    2. investigation of how the topological organization of T4 lysozyme protein (the order of structural elements along the sequence) affects the coupling and folding cooperativity between its domains PMID: 20495548
    3. the lysozyme is inhibited before gp5 is cleaved and, presumably, the lysozyme is activated only after gp5 has penetrated the outer membrane in the infection of E coli PMID: 15701513
    4. When heated, the resulting (gp5*)3 dissociates into three gp5* and a c-terminal trimeric beta-helical structure. The interaction between gp27 and gp5* during infection is sundered by reducing pH. PMID: 16956798
    5. The paper shows that the protein is flexible and adapts to the size and shape of the halogenated benzene ligands. PMID: 19014950

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  • 亚细胞定位：
    [Baseplate central spike protein Gp5*]: Virion.; [Gp5C]: Virion.; [Pre-baseplate central spike protein Gp5]: Virion.
  • 蛋白家族：
    Glycosyl hydrolase 24 family
  • 数据库链接：

    KEGG: vg:1258817

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