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  4. Recombinant Drosophila melanogaster Protein trapped in endoderm-1 (Tre1), partial

Recombinant Drosophila melanogaster Protein trapped in endoderm-1 (Tre1), partial

  • 中文名称：
    黑腹果蝇Tre1重组蛋白
  • 货号：
    CSB-YP868252DLU1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇Tre1重组蛋白
  • 货号：
    CSB-EP868252DLU1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇Tre1重组蛋白
  • 货号：
    CSB-EP868252DLU1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇Tre1重组蛋白
  • 货号：
    CSB-BP868252DLU1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇Tre1重组蛋白
  • 货号：
    CSB-MP868252DLU1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Tre1
  • Uniprot No.：
    Q9NDM2
  • 别名：
    Tre1; CG3171; Protein trapped in endoderm-1
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

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Tre1 Proteins

靶点详情

  • 功能：
    Essential for the first active step of germ cell migration: transepithelial migration of germ cells through the posterior midgut (PMG) epithelium.
  • 基因功能参考文献：
    1. Bifurcation of the Tre1 G protein-coupled receptor signaling response via G protein-dependent and independent branches enables distinct spatiotemporal regulation of germ cell migration. PMID: 28687666
    2. feminizing the Tre1-expressing neurons in male flies leads to rapid courtship initiation, a phenotype that leads to increased reproductive success; Tre1 is expressed in a sexually dimorphic pattern in olfactory sensory neurons and the central nervous system PMID: 26721856
    3. role of the arginine of the NRY motif in Tre1 was investigated; results suggest that a salt bridge may form between this critical arginine and an aspartic acid in transmembrane alpha-helix (TM6)in Tre1 PMID: 24044607
    4. The scattershot allele results in an in-frame deletion of 8 amino acids at the junction of the third transmembrane domain and the second intracellular loop of Tre1 that dramatically impairs the function of this GPCR in germ cell migration. PMID: 20676220
    5. plays an important role in regulation of germ cell migration, polarity , cell adhesion, and cadherin binding. (review) PMID: 20058705
    6. Tre1 is a G protein-coupled receptor that directs transepithelial migration of Drosophila germ cells PMID: 14691551
    7. Down-regulation of E-cadherin causes germ cell dispersal but is not sufficient for transepithelial migration in the absence of Tre1, suggesting a new mechanism for Tre1 GPCR function that links cell polarity, modulation of cell adhesion, and invasion. PMID: 18824569

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 组织特异性：
    In embryos, expression is seen at highest levels in the cuprophilic cells and at lower levels in the amnioserosa, developing CNS, cardiac mesoderm primordium and midline glia.
  • 数据库链接：

    KEGG: dme:Dmel_CG3171

    STRING: 7227.FBpp0070813

    UniGene: Dm.18422

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