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  4. Recombinant Drosophila melanogaster Ecdysone receptor (EcR), partial

Recombinant Drosophila melanogaster Ecdysone receptor (EcR), partial

  • 中文名称：
    黑腹果蝇EcR重组蛋白
  • 货号：
    CSB-YP335887DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇EcR重组蛋白
  • 货号：
    CSB-EP335887DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇EcR重组蛋白
  • 货号：
    CSB-EP335887DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇EcR重组蛋白
  • 货号：
    CSB-BP335887DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇EcR重组蛋白
  • 货号：
    CSB-MP335887DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    EcR
  • Uniprot No.：
    P34021
  • 别名：
    EcR; NR1H1; CG1765Ecdysone receptor; 20-hydroxy-ecdysone receptor; 20E receptor; EcRH; Ecdysteroid receptor; Nuclear receptor subfamily 1 group H member 1
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor for ecdysone. Binds to ecdysone response elements (ECRES) following ecdysone-binding, and recruitment of a complex containing the histone methyltransferase trr, leads to activate transcription of target genes.
  • 基因功能参考文献：
    1. study identifies a non-canonical EcR-corepressor complex with the potential for a direct regulation of ATP-dependent nucleosome remodelling by a nuclear hormone receptor PMID: 28378812
    2. Results shows that ectopic overexpression of mir34 downregulates EcR in mushroom body (MB) neurons suggesting that EcR expression may be under the regulation of mir34 in drosophila. PMID: 28008974
    3. The ecdysone receptor (EcR), which is responsible for initiating the larval-to-adult transition, colocalized with 14-3-3epsilon in wild-type fat body tissues. PMID: 28365999
    4. Polytene chromosome stains showed that Cg co-localizes with EcR, the major regulator of BR-C, at the BR-C locus and that EcR binding to chromatin was sensitive to changes in Cg levels PMID: 27846223
    5. Data show that ecdysone receptors EcRA and EcRB1 are expressed by third instar larvae in the primary nociceptor neurons. PMID: 26495837
    6. CDK8-CycC may serve as transcriptional cofactors for EcR-dependent transcription. PMID: 26222308
    7. E75 and ecdysone receptor are expressed in central clock cells, and manipulations of their expression produce drastic effects on circadian rhythms in Drosophila. PMID: 25511299
    8. Expression levels of estrogen-related receptor gene (ERR), ecdysone receptor gene (EcR) and Yolk protein receptor gene (YPR) were significantly changed by ethylparaben treatment. Female fruit flies are more sensitive to EP than males. PMID: 25265034
    9. B2 isoform of ecdysone receptor is critical for the development of Malpighian tubules and is expressed from early stages of its development. PMID: 25476260
    10. identify a previously uncharacterized enzyme of ecdysone biosynthesis, GstE14, and find that ecdysone triggers pri expression to define the onset of epidermal trichome development PMID: 25344753
    11. EcR signaling modulates the effects of EGFR signaling by promoting an undifferentiated state in early stage testicular cyst cells. PMID: 25169192
    12. Non-conserved residues within the alpha-helix 2, forming the EcRDBD hydrophobic core, represent a specific structural element that contributes to instability. PMID: 24465866
    13. EcR genetically interacts with the NURF chromatin-remodeling complex, which maintains cyst stem cells. ecdysone signaling acts through distinct cell types and effectors to ensure both ovarian and testis stem cell maintenance. PMID: 25093968
    14. dTDP-43 directly and dramatically increased the expression of the neuronal microtubule-associated protein Map205, resulting in cytoplasmic accumulations of the ecdysteroid receptor. PMID: 23333275
    15. The degradation of EcR isoforms was investigated and evidence that N-terminal degradation is modulated by isoform-specific ubiquitination, is provided. PMID: 22568680
    16. These findings suggest that EcR-mediated ecdysone signal is significant in lipid metabolism in insects. PMID: 22503687
    17. Hormonal activation of let-7-C microRNAs via EcR is required for adult Drosophila melanogaster morphology and function. PMID: 22510985
    18. Receptor protein concentration is an important factor that influences ecdysteroid receptor activity. PMID: 21895819
    19. Pzg is associated with EcR in vivo and required for the transcriptional induction of EcR target genes, whereas reduced ecdysteroid levels imply a NURF-independent function of Pzg. PMID: 21385730
    20. EcR-A is required in fru P1-expressing neurons for wild-type male courtship behaviors and the establishment of male-specific neuronal architecture. PMID: 19646872
    21. Spatial patterns of ecdysteroid receptor activation during the onset of Drosophila metamorphosis. PMID: 11923209
    22. every isoform supports development in many tissues but only EcR-A can support developemnt in the margins of the wing discs and only EcR-B2 can do so in the larval epidermis and border cells of the developing egg chamber PMID: 12466195
    23. flies that are heterozygous for mutations of the ecdysone receptor exhibit increases in life-span and resistance to various stresses, with no apparent deficit in fertility or activity PMID: 12610309
    24. different effects of the same point mutations on ligand binding to ecdysone receptor indicate that the ligand-binding pocket is modified by heterodimerization PMID: 12674504
    25. Mutation of EcR abolishes the induction of genes encoding factors from the hedgehog, Notch, EGF, dpp, and wingless pathways. PMID: 12852852
    26. We tested for the ability of the different isoforms to rescue the lack of dendritic pruning that is characteristic of mutants lacking both EcR-B1 and EcR-B2 PMID: 12963111
    27. QSAR and CoMFA models for binding of a training set of 66 ecdysteroids and 97 diacylhydrazines to the D. melanogaster ecdysone receptor demonstrate the relative binding orientation of the two ligand families. PMID: 13677482
    28. This study revealed that the ecdysone receptor (EcR/USP) Ultraspiracle heterodimer displays isoform-specific capabilities. PMID: 17201771
    29. Deficiency of the ecdysone receptor system may have a role in courtship initiation by adult male fruit flies. PMID: 17238001
    30. tissue-specific modulation of EcR activity by the Ras signaling pathway refines temporal ecdysone signals that regulate ovarian follicle cell differentiation PMID: 17436275
    31. Indicate that the architecture of the E-domain of EcR is important for nuclear localization even in the absence of a ligand. PMID: 17884338
    32. These findings suggest that DART1, a histone arginine methyltransferase, is a co-repressor of EcR that is indispensable for normal pupal development in the intact fly. PMID: 18468516
    33. Coordinated expression of the glue genes midway through the third instar is mediated by 20-hydroxyecdysone (20E) acting to induce genes of the Broad Complex (BRC) through a receptor that is not an EcR/USP heterodimer. PMID: 18566664
    34. Down-regulation of Notch signaling and activation of ecdysone receptor (EcR) are required for the endocycle/gene amplification switch in these cells. PMID: 18779369
    35. The EcR-B1 isoform is required during early oogenesis for follicle cell survival and disruption of its function causes apoptotic cell death induced by caspase. PMID: 19015542
    36. Ligand binding to ecdysone receptor heterodimers with Usp PMID: 19428996
    37. steroid hormone receptor EcR finely modulates Drosophila lifespan during adulthood in a sex-specific PMID: 19486910
    38. knocking down EcR derepresses genes that are normally repressed by unliganded EcR, thereby allowing wing patterning to progress PMID: 19515698

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Nuclear hormone receptor family, NR1 subfamily
  • 组织特异性：
    Isoform B1 predominates over isoform A in larval tissues, imaginal histoblast nests and midgut islands. Isoform A predominates over B1 in imaginal disks, and the larval prothoracic gland.
  • 数据库链接：

    KEGG: dme:Dmel_CG1765

    STRING: 7227.FBpp0291631

    UniGene: Dm.4159

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