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  4. Recombinant Bacillus subtilis Penicillin-binding protein 1A/1B (ponA), partial

Recombinant Bacillus subtilis Penicillin-binding protein 1A/1B (ponA), partial

  • 中文名称：
    枯草芽孢杆菌ponA重组蛋白
  • 货号：
    CSB-EP331077BRJ
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    ponA
  • Uniprot No.：
    P39793
  • 别名：
    Peptidoglycan TGase (Penicillin-sensitive transpeptidase) (DD-transpeptidase)
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    28.4 kDa
  • 表达区域：
    774-914aa
  • 氨基酸序列
    AVSDDGKSTASTSYEVPKAEDDEDKKDQQQTDDEKQDDEKTQDDTQTDDSQKDDGQTDQDQTDDSTNDQDKKQDNTNTNPSDNNNQDQSNDNDNDNSNNQDTSDGDSNSGKNDSTGSDTNKNKTDTSNKTQTNSSSIEKTN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    13-23 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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ponA Proteins

靶点详情

  • 功能：
    Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (Probable). Required for vegetative growth (Probable). Has a partially redundant function with PBP-2A (pbpA) during spore outgrowth.
  • 基因功能参考文献：
    1. Elimination of PBP1 suppresses the lethality of an mreB null mutation. Polar cell wall bulging is associated with abnormal localization of PBP1. PMID: 19192185
  • 亚细胞定位：
    Cell membrane; Single-pass type II membrane protein. Forespore inner membrane; Single-pass type II membrane protein. Note=Probably found all over the whole cell at low concentrations. Also localizes to the division site in vegetative cells.
  • 蛋白家族：
    Glycosyltransferase 51 family; Transpeptidase family
  • 数据库链接：

    KEGG: bsu:BSU22320

    STRING: 224308.Bsubs1_010100012276

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