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  4. Recombinant Arabidopsis thaliana Translocase of chloroplast 33, chloroplastic (TOC33), partial

Recombinant Arabidopsis thaliana Translocase of chloroplast 33, chloroplastic (TOC33), partial

  • 中文名称：
    拟南芥TOC33重组蛋白
  • 货号：
    CSB-YP521283DOA1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥TOC33重组蛋白
  • 货号：
    CSB-EP521283DOA1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥TOC33重组蛋白
  • 货号：
    CSB-EP521283DOA1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥TOC33重组蛋白
  • 货号：
    CSB-BP521283DOA1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥TOC33重组蛋白
  • 货号：
    CSB-MP521283DOA1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TOC33
  • Uniprot No.：
    O23680
  • 别名：
    TOC33; PPI1; At1g02280; T7I23.11; Translocase of chloroplast 33, chloroplastic; AtToc33; 33 kDa chloroplast outer envelope protein; Plastid protein import 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

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TOC33 Proteins

靶点详情

  • 功能：
    GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis. Binds GTP, GDP, XTP, but not ATP. Probably specialized in the import of nuclear encoded photosynthetic preproteins from the cytoplasm to the chloroplast, especially during early development stages.
  • 基因功能参考文献：
    1. [review] Homodimerization of Toc33 prevents nucleotide exchange, thereby locking the receptor in the guanosine diphosphate (GDP)-loaded state and preventing further activity. PMID: 21568939
    2. Pre-protein binding induces a dissociation of the Toc33 homodimer and results in exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP). PMID: 21434866
    3. Neither nucleotide binding nor dimerization at atToc33 is essential for chloroplast import. PMID: 20444229
    4. The role of Toc33 protein in biological transport of proteins in the protochlorophyllide-dependent pathway in A. thaliana. PMID: 15773849
    5. The data suggest that atToc33 phosphorylation may not play an important role in vivo. PMID: 16412428
    6. Chloroplast biogenesis and photosynthetic activity are impaired in the mutants during the early developmental stage, which is consistent with the requirement of atToc33 in young photosynthetic tissues. PMID: 18054337
    7. Dimerization is independent of nucleotide loading state, based on crystal structures of dimeric Pisum sativum Toc34 and monomeric Arabidopsis thaliana Toc33. PMID: 18400179
    8. Toc33 GTPase homodimerization and protein translocation into chloroplasts are pH sensitive. PMID: 19001421

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  • 亚细胞定位：
    Plastid, chloroplast outer membrane; Single-pass membrane protein. Note=May contain beta barrel transmembrane regions.
  • 蛋白家族：
    TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, AIG1/Toc34/Toc159-like paraseptin GTPase family, TOC34 subfamily
  • 组织特异性：
    Mostly expressed in seedlings and flowers, and, to a lower extent, in roots, stems, and leaves.
  • 数据库链接：

    KEGG: ath:AT1G02280

    STRING: 3702.AT1G02280.1

    UniGene: At.10710

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