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  4. Recombinant Arabidopsis thaliana Ethylene-insensitive protein 2 (EIN2), partial

Recombinant Arabidopsis thaliana Ethylene-insensitive protein 2 (EIN2), partial

  • 中文名称：
    拟南芥EIN2重组蛋白
  • 货号：
    CSB-YP874354DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥EIN2重组蛋白
  • 货号：
    CSB-EP874354DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥EIN2重组蛋白
  • 货号：
    CSB-EP874354DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥EIN2重组蛋白
  • 货号：
    CSB-BP874354DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥EIN2重组蛋白
  • 货号：
    CSB-MP874354DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    EIN2
  • Uniprot No.：
    Q9S814
  • 别名：
    EIN2; CKR1; ORE3; At5g03280; F12E4.10; MOK16.19Ethylene-insensitive protein 2; AtEIN2; EIN-2; Cytokinin-resistant protein AtCKR1; Protein ORESARA 3) [Cleaved into: EIN2-CEND; EIN2C)]
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

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EIN2 antibodies

靶点详情

  • 功能：
    Central factor in signaling pathways regulated by ethylene (ET), and involved in various processes including development, plant defense, senescence, nucleotide sugar flux, and tropisms. Necessary for ethylene-mediated gene regulation, and for the induction of some genes by ozone. Acts downstream of ET receptors, and upstream of ethylene regulated transcription factors. Required for cytokinin-mediated processes. Seems to be implicated in cross-talk between ET, jasmonate and other pathways. Probably not involved in iron uptake. Has a short half-life and undergoes rapid proteasome-mediated turnover in the absence of ethylene. Required for ethylene-induced EIN3 stabilization via proteasomal degradation of EBF1/EBF2 proteins. Regulates the leaf senescence induced by methyl jasmonate, ethylene and abscisic acid (Ref.17, PubMed:15010611). Required during salt stress to confer resistance.; Trafficking signal inducing ethylene response. The nuclear localization is both necessary and sufficient to activate EIN3-mediated transcription and ethylene responses. Necessary and sufficient for 3'-UTR-mediated translational repression of EBF1 and EBF2 mRNAs. Ethylene induces EIN2-CEND to associate with 3' UTRs in cytoplasmic foci and target EBF1/2 mRNAs to cytoplasmic processing-body (P-body). MPK6 regulates the cleavage and nuclear translocation of EIN2-CEND under methyl jasmonate treatment. Required for EIN3 accumulation.
  • 基因功能参考文献：
    1. ENAP1 may potentially mediate ethylene-induced histone acetylation via its interactions with EIN2 C terminus. PMID: 27694846
    2. In the presence of ethylene, EIN2 interacts with ENAP1, elevating the levels of H3K14Ac and H3K23Ac, promoting more EIN3 binding to the targets shared with ENAP1 and resulting in a rapid transcriptional regulation. PMID: 28874528
    3. These results indicate that root responses to 6-pentyl-2H-pyran-2-one involve components of auxin transport and signaling and the ethylene-response modulator EIN2. PMID: 26568541
    4. role in regulating organ growth by restricting cell expansion PMID: 26039475
    5. Study reports another mechanism of EIN2-mediated ethylene signaling, whereby EIN2 imposes the translational repression of EBF1 and EBF2 mRNA and find that the EBF1/2 3' UTRs mediate EIN2-directed translational repression and identify multiple poly-uridylates (PolyU) motifs as functional cis elements of 3' UTRs. PMID: 26496607
    6. Characterization of one of the targets of the translation regulatory machinery, the ethylene signaling component EBF2, indicates that the signaling molecule EIN2 and the nonsense-mediated decay proteins UPFs play a central role in this ethylene-induced translational response. PMID: 26496608
    7. The data provide insight into an EIN2-mediated senescence signalling pathway that coordinates global gene expression during leaf senescence via a gene regulatory network. PMID: 24659488
    8. the ore1 loss-of-function mutants were epistatic to nes1, suggesting the dominant role of ORE1 and the antagonistic role of NES1 during nitric oxide-induced cotyledon senescence in Arabidopsis. PMID: 24336389
    9. EIN2 may be cleaved at alternative positions PMID: 23656878
    10. Interactions between ECIP1 and EIN2 or ethylene receptors regulate ethylene response and stress response. PMID: 21631530
    11. Data indicate that the expression and phosphorylation of ERF110 were regulated by ethylene via both Ethylene-Insensitive2-dependent and -independent pathways, which constitute a dual-and-opposing mechanism for ethylene in the regulation of bolting. PMID: 23188807
    12. study demonstrated that EIN2 harboring a functional nuclear localization signal is subjected to a cleavage event, followed by transporting its carboxyl end (CEND) into the nucleus, where CEND acts to stabilize EIN3 and activate ethylene responses PMID: 23070300
    13. reducing jasmonic acid levels, either mutationally or chemically, caused ein2 ethylene-insensitive mutants to become ethylene responsive. This effect was not seen with the ethylene-insensitive etr1-1 mutants that affect receptor function. PMID: 23219920
    14. involved in nitric oxide signaling in the regulation of leaf senescence PMID: 22765302
    15. These findings uncover a mechanism of subcellular communication whereby ethylene stimulates phosphorylation-dependent cleavage and nuclear movement of the EIN2-C' peptide, linking hormone perception and signaling components in the ER with nuclear-localized transcriptional regulators. PMID: 22936567
    16. These data suggest a novel model on the integration of EIN2 in the ethylene signaling pathway. PMID: 21242723
    17. AtMYB44 regulates induced expression of EIN2 in HrpN(Ea)-treated Arabidopsis plants. AtMYB44 protein localizes to nuclei and binds the EIN2 promoter; HrpN(Ea) treatment promotes AtMYB44 production, binding activity, and transcription of AtMYB44 and EIN2. PMID: 21117868
    18. Data show that protein phosphorylation is a key mechanism to control the interaction of EIN2 and the receptors. PMID: 20591837
    19. The etr1-3 and ein2-1 mutants exhibited less reductions in lateral root length and number than wild-type plants in response to high nitrate concentration. PMID: 19732351
    20. EIN2 membrane protein is required for both the first and second phases of growth inhibition by ethylene in Arabidopsis seedling hypocotyls. PMID: 15466219
    21. RCN1 and EIN2 modulate hypocotyl gravitropism and ethylene responses through independent pathways. PMID: 16798939
    22. EIN2 is a cross-link node in ethylene, ABA and stress signaling pathways, and EIN2 is necessary to induce developmental arrest during seed germination, and seedling establishment, as well as subsequent vegetative growth PMID: 17533512
    23. The results suggest that EIN2 gene mediates Pb(II) resistance, at least in part, through two distinct mechanisms, a GSH-dependent mechanism and a GSH-independent AtPDR12-mediated mechanism. PMID: 19153049
    24. Overexpression of ETP1 or ETP2 disrupts EIN2 protein accumulation, and these plants manifest a strong ethylene-insensitive phenotype PMID: 19196655
    25. ORE1 positively regulates aging-induced cell death; ORE1 expression is upregulated with leaf aging by EIN2 but negatively regulated by miR164; feed-forward pathway involving ORE1, miR164 & EIN2 provides regulation ensuring aging induces cell death PMID: 19229035
    26. These results suggest that ethylene signals may be transduced by a phosphor-relay from receptors to transcriptional events via both ein2-dependent and -independent pathways. PMID: 19253305

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  • 亚细胞定位：
    [Ethylene-insensitive protein 2]: Endoplasmic reticulum membrane; Multi-pass membrane protein.; [EIN2-CEND]: Nucleus. Cytoplasm. Endoplasmic reticulum membrane.
  • 蛋白家族：
    NRAMP (TC 2.A.55) family
  • 组织特异性：
    [EIN2-CEND]: Localized to the guard cells after methyl jasmonate treatment.
  • 数据库链接：

    KEGG: ath:AT5G03280

    STRING: 3702.AT5G03280.1

    UniGene: At.1598

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