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  4. Recombinant Arabidopsis thaliana Elongator complex protein 3 (HAG3), partial

Recombinant Arabidopsis thaliana Elongator complex protein 3 (HAG3), partial

  • 中文名称：
    拟南芥HAG3重组蛋白
  • 货号：
    CSB-YP842426DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥HAG3重组蛋白
  • 货号：
    CSB-EP842426DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥HAG3重组蛋白
  • 货号：
    CSB-EP842426DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥HAG3重组蛋白
  • 货号：
    CSB-BP842426DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥HAG3重组蛋白
  • 货号：
    CSB-MP842426DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HAG3
  • Uniprot No.：
    Q93ZR1
  • 别名：
    HAG3; EAST1; ELO3; ELP3; At5g50320; MXI22.3Elongator complex protein 3; AtELP3; Elongator component 3; Protein ELONGATA 3; Protein ENHANCER-OF-ASYMMETRIC-LEAVES-TWO1; tRNA uridine(34) acetyltransferase; EC 2.3.1.-
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Involved in tRNA wobble uridine modification. In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. Promotes organs development by modulating cell division rate. Required for auxin distribution or signaling. Required for meristem cell cycle activation at the time of germination. Mediates the establishment of leaf polarity independently of AS2 and the ta-siRNA-related pathway. Negatively regulates the expression of genes that participate in UV-B responses, such as DNA repair enzymes and enzymes that participate in sunscreen pigment biosynthesis.
  • 基因功能参考文献：
    1. Data indicate that histone acetyltransferase HAG3, directly or indirectly, participates in UV-B-induced DNA damage repair and signaling. PMID: 25907565
    2. ELONGATA3 (ELO3) as a key regulator of meristem cell cycle activation in Arabidopsis. PMID: 23969312
    3. AtELP3 regulates plant immunity through its histone acetyltransferase and radical S-adenosylmethionine domains. PMID: 23856002
    4. ELO3 mediated establishment of leaf polarity independently of AS2 and the trans-acting small interfering RNA-related pathway. PMID: 21700721
    5. Results conclude that yeast and plant Elongator (elp3/ELO3) share tRNA modification roles and propose that this function might be conserved in Elongator from all eukaryotic kingdoms of life. PMID: 20398216

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    ELP3 family
  • 组织特异性：
    Expressed in meristematic tissues of in roots, leaves, seedlings, cotyledons, floral buds and shoot apices. Expressed in the shoot meristem and the emerging leaves immediately after germination. In the root, expressed in the transition zone and the upper
  • 数据库链接：

    KEGG: ath:AT5G50320

    STRING: 3702.AT5G50320.1

    UniGene: At.20657

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