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  4. Recombinant Arabidopsis thaliana Cystathionine gamma-synthase, chloroplastic (CGS1)

Recombinant Arabidopsis thaliana Cystathionine gamma-synthase, chloroplastic (CGS1)

  • 中文名称：
    Recombinant Arabidopsis thaliana Cystathionine gamma-synthase, chloroplastic(CGS1)
  • 货号：
    CSB-YP347576DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Cystathionine gamma-synthase, chloroplastic(CGS1)
  • 货号：
    CSB-EP347576DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Cystathionine gamma-synthase, chloroplastic(CGS1)
  • 货号：
    CSB-EP347576DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Cystathionine gamma-synthase, chloroplastic(CGS1)
  • 货号：
    CSB-BP347576DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Cystathionine gamma-synthase, chloroplastic(CGS1)
  • 货号：
    CSB-MP347576DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CGS1
  • Uniprot No.：
    P55217
  • 别名：
    CGS1; CYS1; MTO1; At3g01120; T4P13.19Cystathionine gamma-synthase 1; chloroplastic; AtCGS1; EC 2.5.1.48; METHIONINE OVERACCUMULATION 1; O-succinylhomoserine; thiol)-lyase
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    183-563
  • 氨基酸序列
    GERLGRGI VTDAITTPVV NTSAYFFKKT AELIDFKEKR SVSFEYGRYG NPTTVVLEDK ISALEGAEST LVMASGMCAS TVMLLALVPA GGHIVTTTDC YRKTRIFMEN FLPKLGITVT VIDPADIAGL EAAVNEFKVS LFFTESPTNP FLRCVDIELV SKICHKRGTL VCIDGTFATP LNQKALALGA DLVVHSATKY IGGHNDVLAG CICGSLKLVS EIRNLHHVLG GTLNPNAAYL IIRGMKTLHL RVQQQNSTAF RMAEILEAHP KVSHVYYPGL PSHPEHELAK RQMTGFGGVV SFEIDGDIET TIKFVDSLKI PYIAPSFGGC ESIVDQPAIM SYWDLPQEER LKYGIKDNLV RFSFGVEDFE DVKADILQAL EAI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the first committed step of methionine (Met) biosynthesis. Catalyzes the formation of L-cystathionine from homoserine esters and L-cysteine, via a gamma-replacement reaction. Substrate preference for cystathionine synthesis is O-phospho-L-homoserine (OPH) > O(4)-succinyl-L-homoserine (OSH) >> O-acetyl-L-homoserine (OAH). Is able, at extremely low rate, to catalyze a gamma-elimination of OPH in the absence of cysteine to produce inorganic phosphate (Pi), 2-oxobutanoate and ammonia.
  • 基因功能参考文献：
    1. Seed-specific repression of AtCGS in RNA interference seeds triggered the induction of genes operating in the SMM cycle of rosette leaves. PMID: 28536103
    2. Cystathione-gamma-synthase stimulates metabolic and transcriptomic responses associated with desiccation stress in seeds. PMID: 25232013
    3. Report shows that the poly(A) tail of the full-length CGS1 mRNA showed an apparent increase from 50-80 nucleotides (nt)to 140-150 nt after the induction of CGS1 mRNA degradation. PMID: 24463527
    4. Data indicate that stacked ribosomes play in the control of cystathionine gamma-synthase CGS1 mRNA degradation. PMID: 24652291
    5. Light and sucrose up-regulate the expression level of cystathionine gamma-synthase. PMID: 23933995
    6. The transcript of the N-terminus region deleted form of CGS is insensitive to methionine application and its expression may be important for maintaining methionine metabolism even in the presence of a high level of methionine. PMID: 16507086
    7. The results of this study suggest that a metabolic effector, to date unidentified, can modulate CGS activity in vivo through an interaction with the N-terminal domain of the enzyme and that removal of this domain can suppress this regulation. PMID: 17720756
    8. These results show that S-adenosyl-L-methionine induces the stacking of ribosomes on CGS1 mRNA and that multiple mRNA degradation sites probably correspond to each stacked ribosome. PMID: 18184691
    9. CGS1 mRNA degradation involves a plant-specific mechanism. PMID: 18285355

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  • 亚细胞定位：
    Plastid, chloroplast.
  • 蛋白家族：
    Trans-sulfuration enzymes family
  • 数据库链接：

    KEGG: ath:AT3G01120

    STRING: 3702.AT3G01120.1

    UniGene: At.20198

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