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  4. Phospho-GYS1 (S641) Recombinant Monoclonal Antibody

Phospho-GYS1 (S641) Recombinant Monoclonal Antibody

文献引用 (1) 说明书
  • 中文名称：
    磷酸化-GYS1 (S641)重组抗体
  • 货号：
    CSB-RA010078A641phHU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in Hela whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or EGF)
      All lanes Phospho-GYS1 antibody at 1.12μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 85 KDa
      Observed band size: 85 KDa
    • Immunofluorescence staining of HepG2 cells with CSB-RA010078A641phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

产品详情

  • 产品描述：
    Phospho-GYS1 (S641) Recombinant Monoclonal Antibody（Code: CSB-RA010078A641phHU）是一款高特异性抗体，靶向糖原合成酶1（GYS1）第641位丝氨酸磷酸化修饰位点。GYS1是糖原合成的关键限速酶，其活性通过S641等位点的磷酸化受到精确调控，这一修饰可抑制酶活性，进而影响细胞能量代谢稳态，在糖尿病、肿瘤等疾病的代谢重编程研究中具有重要意义。该抗体经ELISA、Western Blot（WB）及免疫荧光（IF）等多平台验证，可特异性识别内源性磷酸化GYS1蛋白，推荐稀释度为WB 1:500-1:5000、IF 1:20-1:200，批次间一致性优异。适用于研究能量代谢调控机制、糖原合成通路异常相关疾病模型及药物干预下的磷酸化动态变化，为探索细胞葡萄糖代谢、胰岛素信号传导及病理状态下代谢适应性改变提供可靠工具。本产品仅用于科学研究，不适用于临床诊断或其他用途。
  • Uniprot No.：
    P13807
  • 基因名：
    GYS1
  • 别名：
    Glycogen [starch] synthase antibody; Glycogen synthase 1 (muscle) antibody; Glycogen synthase 1 antibody; GSY antibody; GYS antibody; Gys1 antibody; GYS1_HUMAN antibody; muscle antibody
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from Human Phospho-GYS1 (S641)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    1D1
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:20-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunofluorescence (IF) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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GYS1 antibodies

GYS1 Proteins

靶点详情

  • 功能：
    Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
  • 基因功能参考文献：
    1. Insulin-stimulated glycogen synthase (GS) activity was completely ablated during hyperinsulinemic hypoglycemia, and catecholamine signaling via cAMP-dependent protein kinase (PKA) and phosphorylation of inhibiting sites on GS all increased. PMID: 28596236
    2. Overexpression of GYS1, MIF, and MYC is associated with adverse outcome and poor response to azacitidine in myelodysplastic syndromes and acute myeloid leukemia PMID: 25487600
    3. over-expression of muscle glycogen synthase (MGS)was detected in diabetic human kidney PMID: 25371328
    4. High glycogen synthase 1 expression is associated with myeloid leukemia. PMID: 25703587
    5. Data suggest that although COOH-terminal dephosphorylation is likely necessary for GS activation, protein kinase Akt-2- (Akt2)-dependent NH2-terminal dephosphorylation is site for "fine-tuning" insulin-mediated GS activation in skeletal muscle. PMID: 23321478
    6. Allosteric regulation and the relationship between phosphorylation and the kinetics of glycogen synthase. [Review] PMID: 23134486
    7. The present findings demonstrate that physical inactivity-induced insulin resistance in muscle is associated with lower content/activity of key proteins in glucose transport/phosphorylation and storage. PMID: 22403297
    8. Exercise unmaskd the effect associated with the GYS1 polymorphism, rendering carriers of this allele less susceptible to the protective effect of exercise on the risk of cardiovascular mortality. PMID: 17356695
    9. the M416V polymorphism of glycogen synthase 1 gene is not associated with insulin resistance in type 2 diabetes PMID: 12870167
    10. Phosphorylation at site 2 in the elderly and at site 3a + 3b in young twins had a genetic component. PMID: 15855312
    11. no nuclear export signal was identified in the sequence of the protein, the region comprising amino acids 555-633, which has an Arg-rich cluster involved in the allosteric activation is crucial for its nuclear concentration and aggregation. PMID: 15955076
    12. 3 siblings were identified with profound muscle glycogen deficiency and homozygous stop mutations in GYS1 PMID: 17928598
    13. Pioglitazone treatment improved insulin-stimulated glucose metabolism and glycogen synthase activity in PCOS. PMID: 18544618
    14. After overnight low muscle glycogen level and/or in response to exhausting exercise-induced glycogenolysis, GSY is associated with spherical structures at the I-band of sarcomeres. PMID: 19339242
    15. Dysregulation of glycogen synthase phosphorylation plays a major role in impaired insulin regulation of GS in obesity and type 2 diabetes mellitus. PMID: 19837931

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  • 相关疾病：
    Muscle glycogen storage disease 0 (GSD0b)
  • 蛋白家族：
    Glycosyltransferase 3 family
  • 数据库链接：

    HGNC: 4706

    OMIM: 138570

    KEGG: hsa:2997

    STRING: 9606.ENSP00000317904

    UniGene: Hs.386225

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