TPSB2 Recombinant Monoclonal Antibody

中文名称：

TPSB2重组抗体
货号：

CSB-RA958088A0HU
规格：

￥1320
图片：
Western Blot
Positive WB detected in: Mouse Kidney tissue lysate,
All lanes: TPSB2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa

Overlay Peak curve showing K562 cells stained with CSB-RA958088A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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产品描述：

CSB-RA958088A0HU TPSB2重组单克隆抗体是针对人源类胰蛋白酶β2（Tryptase Beta-2）研发的高特异性检测工具，适用于多种实验场景。TPSB2基因编码的丝氨酸蛋白酶主要储存于肥大细胞颗粒中，参与调控炎症反应、组织修复及免疫应答等生理病理过程，其异常表达与过敏性疾病、哮喘等炎症相关疾病机制密切相关。本抗体经严格验证，在Western Blot中可清晰识别内源性TPSB2蛋白，推荐使用稀释比例为1:500-1:2000；流式细胞分析（FC）应用中建议稀释比例为1:50-1:200，同时支持ELISA检测，展现优异的批次稳定性和抗原结合能力。该产品适用于肥大细胞功能研究、炎症信号通路解析及疾病模型构建等科研领域，为探索免疫调控机制和药物靶点筛选提供可靠工具，所有应用仅限于科学研究用途。
Uniprot No.：

P20231
基因名：

TPSB2
别名：

Tryptase beta-2 (Tryptase-2) (EC 3.4.21.59) (Tryptase II), TPSB2, TPS2
反应种属：

Human, Mouse
免疫原：

A synthesized peptide derived from Human TPSB2
免疫原种属：

Homo sapiens (Human)
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

Rabbit IgG
纯化方式：

Affinity-chromatography
克隆号：

9H6
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
产品提供形式：

Liquid
应用范围：

ELISA, WB, FC
推荐稀释比：

Application Recommended Dilution

WB 1:500-1:2000

FC 1:50-1:200
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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功能：

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
基因功能参考文献：
1. These observations indicate that betaII-tryptase activity is post-translationally regulated by an allosteric disulfide bond. PMID: 24142694
2. Results indicate a key role for heparin in the activation of human betaI- and beta2-tryptase. PMID: 15567416
3. The disease severity and plasma tryptase levels were not affected by the number of alpha or beta tryptase alleles in mastocytosis patients. PMID: 17449330
4. Strong linkage of TPSAB1 and TPSB2 and pairing of deficiency alleles with functional alleles in observed haplotypes protect human subjects from "knockout" genomes and indeed from inheritance of fewer than 2 active alleles. PMID: 19748655
亚细胞定位：

Secreted. Note=Released from the secretory granules upon mast cell activation.
蛋白家族：

Peptidase S1 family, Tryptase subfamily
数据库链接：

HGNC: 14120

OMIM: 191081

KEGG: hsa:64499

UniGene: Hs.405479

TPSB2 Recombinant Monoclonal Antibody

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Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200