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  4. PIN4 Recombinant Monoclonal Antibody

PIN4 Recombinant Monoclonal Antibody

说明书
  • 中文名称：
    PIN4重组抗体
  • 货号：
    CSB-RA916699A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: U251 whole cell lysate, MCF-7 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate
      All lanes: PIN4 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 14, 17, 15 kDa
      Observed band size: 12-17 kDa
    • IHC image of CSB-RA916699A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of MCF-7 cell with CSB-RA169000A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 598-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：
    CSB-RA916699A0HU重组单克隆抗体是针对PIN4蛋白研发的高特异性试剂，适用于多种蛋白检测场景。PIN4（Peptidyl-prolyl cis/trans isomerase）属于肽酰脯氨酰顺反异构酶家族，通过调控磷酸化蛋白的构象变化参与细胞周期调控、应激响应及肿瘤发生等过程，在癌症与神经退行性疾病研究中备受关注。本抗体经ELISA、Western Blot（WB）、免疫组化（IHC）和免疫荧光（IF）多平台验证，在WB实验中可清晰识别天然蛋白，IHC显示组织定位特异性，IF检测呈现亚细胞水平的高分辨率信号，推荐稀释度分别为WB 1:500-1:2000、IHC 1:50-1:200、IF 1:50-1:200。其低交叉反应性和高批次稳定性使其成为研究PIN4在细胞周期异常、蛋白折叠障碍及相关疾病分子机制中的理想工具，适用于肿瘤生物学、神经病理学及基础蛋白质相互作用研究领域。
  • Uniprot No.：
    Q9Y237
  • 基因名：
    PIN4
  • 别名：
    Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (EC 5.2.1.8) (Parvulin-14) (Par14) (hPar14) (Parvulin-17) (Par17) (hPar17) (Peptidyl-prolyl cis-trans isomerase Pin4) (PPIase Pin4) (Peptidyl-prolyl cis/trans isomerase EPVH) (hEPVH) (Rotamase Pin4), PIN4
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human PIN4
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    14A7
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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PIN4 antibodies

靶点详情

  • 功能：
    Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.; Isoform 2 binds to double-stranded DNA.
  • 基因功能参考文献：
    1. Par14 can be described as an endogenous non-histone chromatin protein, which binds DNA in vivo PMID: 25645591
    2. Ca(2+)/Calmodulin binding to the N-terminal of Par17 causes steric hindrance of the Par17 active site, thus interfering with the Par17/tubulin interaction. PMID: 25940090
    3. Data indicate that Pin1 prolyl isomerase active site cysteine, Cys113, is highly susceptible to oxidation. PMID: 25595659
    4. Data indicate the association of Par14 with insulin receptor substrate 1 (IRS-1) in human HepG2 cells overexpressing both as well as endogenously in the mouse liver. PMID: 23720771
    5. The N-terminal basic domain of human parvulin hPar14 is responsible for the entry to the nucleus and high-affinity DNA-binding. PMID: 12144781
    6. subcellular localization and DNA binding properties of hPar14 are regulated by phosphorylation of the N-terminal domain PMID: 12860119
    7. Identification of a longer Parvulin isoform (Par17) that has an extension at the 5' end including a 75 bp extended open reading frame. PMID: 16522211

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  • 亚细胞定位：
    [Isoform 1]: Nucleus, nucleolus. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Note=Colocalizes in the nucleolus during interphase and on the spindle apparatus during mitosis with NPM1.; [Isoform 2]: Mitochondrion. Mitochondrion matrix. Note=Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide.
  • 蛋白家族：
    PpiC/parvulin rotamase family, PIN4 subfamily
  • 组织特异性：
    Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much m
  • 数据库链接：

    HGNC: 8992

    OMIM: 300252

    KEGG: hsa:5303

    STRING: 9606.ENSP00000362773

    UniGene: Hs.655623

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