Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. Home
  2. 抗体
  3. 多克隆抗体
  4. PIN4 Antibody

PIN4 Antibody

说明书
  • 中文名称：
    PIN4兔多克隆抗体
  • 货号：
    CSB-PA861478LA01HU
  • 规格：
    ￥440
  • 图片：
    • Western Blot
      Positive WB detected in: 293T whole cell lysate(30µg),MCF7 whole cell lysate(30µg), 293 whole cell lysate(30µg)
      All lanes: PIN4 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/20000 dilution
      Predicted band size: 14, 17,15 kDa
      Observed band size: 14 kDa
      Exposure time:120s
    • IHC image of CSB-PA861478LA01HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • IHC image of CSB-PA861478LA01HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • IHC image of CSB-PA861478LA01HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancerperformed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • Immunofluorescence staining of MCF7 cell with CSB-PA861478LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of MCF7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of HepG2 cell with CSB-PA861478LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PIN4 Polyclonal antibody
  • Uniprot No.：
    Q9Y237
  • 基因名：
    PIN4
  • 别名：
    EPVH antibody; Eukaryotic parvulin homolog antibody; hEPVH antibody; hPar14 antibody; hPar17 antibody; MGC138486 antibody; OTTHUMP00000023527 antibody; OTTHUMP00000217483 antibody; OTTHUMP00000217485 antibody; Par14 antibody; Par17 antibody; Parvulin 14 antibody; Parvulin-14 antibody; Parvulin-17 antibody; Peptidyl prolyl cis trans isomerase NIMA interacting 4 antibody; Peptidyl prolyl cis/trans isomerase EPVH antibody; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 antibody; Peptidyl-prolyl cis-trans isomerase PIN4 antibody; Peptidyl-prolyl cis/trans isomerase EPVH antibody; PIN4 antibody; PIN4_HUMAN antibody; PPIase Pin4 antibody; Protein (peptidylprolyl cis/trans isomerase) NIMA interacting, 4 (parvulin) antibody; Rotamase Pin4 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from human PIN4
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:3000
    IHC 1:100-1:300
    IF 20-1:100
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

产品评价

相关产品

PIN4 antibodies

靶点详情

  • 功能：
    Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.; Isoform 2 binds to double-stranded DNA.
  • 基因功能参考文献：
    1. Par14 can be described as an endogenous non-histone chromatin protein, which binds DNA in vivo PMID: 25645591
    2. Ca(2+)/Calmodulin binding to the N-terminal of Par17 causes steric hindrance of the Par17 active site, thus interfering with the Par17/tubulin interaction. PMID: 25940090
    3. Data indicate that Pin1 prolyl isomerase active site cysteine, Cys113, is highly susceptible to oxidation. PMID: 25595659
    4. Data indicate the association of Par14 with insulin receptor substrate 1 (IRS-1) in human HepG2 cells overexpressing both as well as endogenously in the mouse liver. PMID: 23720771
    5. The N-terminal basic domain of human parvulin hPar14 is responsible for the entry to the nucleus and high-affinity DNA-binding. PMID: 12144781
    6. subcellular localization and DNA binding properties of hPar14 are regulated by phosphorylation of the N-terminal domain PMID: 12860119
    7. Identification of a longer Parvulin isoform (Par17) that has an extension at the 5' end including a 75 bp extended open reading frame. PMID: 16522211

    显示更多

    收起更多

  • 亚细胞定位：
    [Isoform 1]: Nucleus, nucleolus. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Note=Colocalizes in the nucleolus during interphase and on the spindle apparatus during mitosis with NPM1.; [Isoform 2]: Mitochondrion. Mitochondrion matrix. Note=Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide.
  • 蛋白家族：
    PpiC/parvulin rotamase family, PIN4 subfamily
  • 组织特异性：
    Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much m
  • 数据库链接：

    HGNC: 8992

    OMIM: 300252

    KEGG: hsa:5303

    STRING: 9606.ENSP00000362773

    UniGene: Hs.655623

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |