Sheep ghrelin/obestatin prepropeptide (GHRL) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

绵羊饥饿激素(GHRL)酶联免疫试剂盒（CSB-EL009414SH）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GHRL含量。靶点GHRL即胃饥饿素，它是一种主要由胃分泌的肽类激素。在研究机制方面，它与调节食欲、能量平衡及生长激素释放等相关。其含量变化影响机体代谢，当下围绕它在肥胖、糖尿病等疾病中的作用及潜在治疗价值展开诸多研究。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，其应用场景涵盖绵羊营养代谢研究、能量稳态相关疾病模型的分子机制解析，以及GHRL在胃肠道功能调控中的作用评估等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

GHRL
种属：

Ovis aries (Sheep)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-98
1:2	Average %	97
	Range %	92-102
1:4	Average %	100
	Range %	94-106
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of sheep GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	95-105
EDTA plasma (n=4)	94	89-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.