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中文名称：猪生饥饿激素(GHRL)酶联免疫试剂盒
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货号：CSB-E09437p
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规格：96T/48T
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价格：￥4200/￥3000
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其他：
产品详情
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产品描述：猪生饥饿激素(GHRL)酶联免疫试剂盒（CSB-E09437p）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GHRL含量。GHRL即胃饥饿素，是胃分泌的一种肽。它能调节食欲和能量稳态，与肥胖、糖尿病等代谢疾病相关。研究机制主要围绕其与受体的结合，通过信号传导调节神经内分泌，影响进食行为和代谢过程，为相关疾病治疗提供潜在靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，本试剂盒适用于科研场景中GHRL相关基础研究，例如探索营养干预、代谢性疾病模型或生理周期变化对GHRL表达的影响，满足能量代谢机制、内分泌调控通路等领域的科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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别名：GHRLAppetite-regulating hormone ELISA Kit; Growth hormone secretagogue ELISA Kit; Growth hormone-releasing peptide ELISA Kit; Motilin-related peptide) [Cleaved into: Ghrelin; Obestatin] ELISA Kit
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缩写：
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Uniprot No.：
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种属：Sus scrofa (Pig)
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样本类型：serum, plasma, tissue homogenates
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检测范围：62.5 pg/mL-4000 pg/mL
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灵敏度：15.6 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Cardiovascular
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of pig GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 89-97 1:2 Average % 99 Range % 94-103 1:4 Average % 106 Range % 100-114 1:8 Average % 95 Range % 90-100 -
回收率：
The recovery of pig GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 84 80-88 EDTA plasma (n=4) 88 84-93 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 4000 2.481 2.583 2.532 2.382 2000 1.873 1.904 1.889 1.739 1000 1.293 1.221 1.257 1.107 500 0.734 0.708 0.721 0.571 250 0.427 0.462 0.445 0.295 125 0.322 0.302 0.312 0.162 62.5 0.250 0.259 0.255 0.105 0 0.154 0.146 0.150 -
数据处理：
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货期：3-5 working days
引用文献
- Effects of Moringa oleifera Leaf Meal on Plasma Ghrelin and Insulin-like Growth Factor-1 Levels in Swine and its Potential Role in Improving Sow Productivity RKEB Gonzales,Philippine Agricultural,2020
- New polymorphisms and expression of the porcine ghrelin (GHRL) gene in different pig breeds Y. Wang et al,Journal of animal and feed sciences,2012
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靶点详情
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功能：Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.; Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
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基因功能参考文献：
- These expression data may indicate that factors other than ghrelin and leptin are more involved in the regulation of feed intake in pigs apparently acclimated to heat stress. PMID: 27264891
- slc30A8 colocalizes with ghrelin and motilin in the gastrointestinal tract of pigs PMID: 26388512
- Studied an alternative vascular approach to the modulation of gastric ghrelin levels.. PMID: 25294792
- Obestatin enhances proliferation and differentiation of preadipocytes promoting PPARgamma and C/EBPa expression, and inhibiting preadipocyte apoptosis by decreasing expression of Caspase-3, Caspase-7 and Caspase-9. PMID: 24534601
- The expression of ghrelin in the digestive tract of low birth weight and normal weight piglets is reported. PMID: 24237675
- Data suggest that a dietary factor (here, high levels of dietary copper) is able to up-regulate expression of ghrelin in the fundic gland of growing pigs; the diets tested in this study also increase feed intake and promote weight gain. PMID: 22911428
- Ghrelin immunolocalization in the gastrointestinal tract of pigs at different ages were studied and it was concluded that gastric ghrelin expression is not related merely to age but could also potentially be influenced by food intake. PMID: 22281862
- BsrI polymorphism at the ghrelin gene locus is potentially associated with carcass and meat quality traits. PMID: 21669497
- Plasma concentrations of ghrelin were greater before feeding and decreased after feeding in pigs fed once per day; there was no consistent pattern to ghrelin secretion in the ad libitum fed group. PMID: 20399063
- glucose output by primary porcine hepatocytes in suspension culture, after incubation with acylated ghrelin (AG), unacylated ghrelin (UAG), and hexarelin (HEX). PMID: 15536157
- Immunization against ghrelin was associated with increased antibody titers, decreased voluntary food consumption, and decreased body weight gain by pigs. PMID: 16793235
- ghrelin mRNA expression is up-regulated 10 days after weaning in the gastric fundus of piglets, coinciding with the increase of H(+)-K(+)-ATPase mRNA expression and activity PMID: 16920167
- growth hormone stimulated both ghrelin synthesis and secretion in the ovarian follicles PMID: 17951088
- Ghrelin requires activation of the NOS/NO route, and its subsequent GC/cGMP signal transduction pathway, as necessary steps to induce GH secretion from somatotrophs. PMID: 18208548
- Expression of ghrelin mRNA is affected by estrus in the hypothalamus, pituitary gland and ovary. PMID: 18295988
- circulating total plasma ghrelin concentrations in the pig appear to be influenced by chronic changes in energy balance rather than the feeding pattern per se. PMID: 18434347
- provides novel evidence that ghrelin has a modulatory effect in the ovary PMID: 19212011
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亚细胞定位：Secreted.
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蛋白家族：Motilin family
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数据库链接：
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