Sheep arachidonic Acid(AA) ELISA kit

产品详情

产品描述：

CUSABIO绵羊花生四烯酸（AA）酶联免疫检测试剂盒（货号：CSB-EQ027590SH），采用竞争法原理实现定量分析，适用于血清、血浆样本中AA含量的精准检测。花生四烯酸作为重要的ω-6多不饱和脂肪酸，广泛参与炎症反应、细胞信号转导及脂质代谢等生理过程，是研究免疫调节、心血管功能及代谢性疾病的关键分子。本试剂盒检测范围为15.625 ng/mL至1000 ng/mL，通过优化的抗体-抗原竞争反应体系，可灵敏识别样本中的AA分子，操作流程符合常规ELISA实验标准。该产品适用于科研领域中对绵羊模型炎症机制、营养代谢调控、药物干预效果的评价，以及脂质代谢相关通路的基础研究，为体外实验提供可靠的定量数据支持，实验全程需严格参照说明书进行样本预处理和标准曲线建立。
缩写：

AA
种属：

Ovis aries (Sheep)
样本类型：

serum, plasma
检测范围：

15.625 ng/mL-1000 ng/mL
灵敏度：

13.797 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Biochemicals
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	89-100
1:200	Average %	90
	Range %	85-95
1:400	Average %	100
	Range %	94-104
1:800	Average %	105
	Range %	97-110

回收率：

The recovery of sheep AA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	95-106
EDTA plasma (n=4)	102	96-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.