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中文名称：人花生四烯酸(AA)酶联免疫试剂盒
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货号：CSB-E09040h
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：CUSABIO人花生四烯酸（Arachidonic Acid, AA）酶联免疫检测试剂盒（货号：CSB-E09040h），采用竞争法定量原理，可精准测定血清、血浆、细胞培养上清及组织匀浆样本中AA的浓度。花生四烯酸作为重要的多不饱和脂肪酸，是前列腺素、白三烯等炎症介质的前体分子，广泛参与细胞信号传导、免疫调节及脂质代谢等生物学过程。本试剂盒检测范围为78.125 ng/mL至5000 ng/mL，通过预包被的AA结合物与样本中游离AA竞争结合特异性抗体，结合辣根过氧化物酶标记的二抗催化显色反应，实现目标物的定量分析。试剂盒包含预包被96孔板、标准品、检测抗体、显色底物及终止液等即用型组分，适用于基础科研中炎症机制研究、脂质代谢调控实验、药物或营养干预对AA通路影响的评估，以及细胞模型中AA释放水平的动态监测。该检测体系具备高灵敏度与特异性，操作流程标准化，可为探索AA在心血管疾病、神经退行性疾病等病理生理过程中的作用提供可靠工具。
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别名：N/A
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缩写：AA
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种属：Homo sapiens (Human)
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样本类型：serum, plasma, cell culture supernates, tissue homogenates
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检测范围：78.125 ng/mL-5000 ng/mL
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灵敏度：69.748 ng/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Biochemicals
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测定原理：quantitative
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测定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
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数据处理：
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货期：3-5 working days
引用文献
- Essential roles of exosome and circRNA_101093 on ferroptosis desensitization in lung adenocarcinoma X Zhang,Cancer communications,2023
- Aqueous Extract of Tournefortia sarmentosa Stem Inhibits ADP-induced Platelet Aggregation Wang, L.et al, Indian Journal of Pharmaceutical Sciences ,2017
- Inhibitory Effects of Total Saponin Korean Red Ginseng on Thromboxane A Jung-Hae Shin.et al, The Korean Society For Biomedical Laboratory Sciences,2017
- Inhibitory effects of thromboxane A2 generation by ginsenoside Ro due to attenuation of cytosolic phospholipase A2 phosphorylation Jung-Hae Shin.et al,Journal of Ginseng Research,2018
- Surfactant Protein B Suppresses Lung Cancer Progression by Inhibiting Secretory Phospholipase A2 Activity and Arachidonic Acid Production Lee S.et al,Cell Physiol Biochem,2017
- Antipsychotic Drugs Inhibit Platelet Aggregation via P2Y1 and P2Y12 Receptors Chang-Chieh Wu.et al,BioMed Research International,2016
- H-rev107 Regulates Cytochrome P450 Reductase Activity and Increases Lipid Accumulation Tsai FM. et al,PLoS One,2015
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靶点详情
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最新研究进展：近年来，花生四烯酸（Arachidonic Acid，AA）的研究重点主要集中在其与疾病的关系、作用机制以及调节方法等方面。一些研究表明，花生四烯酸的代谢异常与多种疾病密切相关。例如，心血管疾病的发生与发展与花生四烯酸的代谢异常有关，AA通过氧化代谢生成的炎症介质，如前列腺素和白三烯等，可导致血管内皮损伤、炎症反应和血栓形成等。同时，花生四烯酸在肿瘤发生和发展中也发挥了一定的作用。花生四烯酸代谢产物可以促进肿瘤细胞增殖、侵袭和转移，并在肿瘤的血管生成过程中发挥一定的作用。研究人员可通过调节花生四烯酸的代谢途径，探索其在疾病预防和治疗方面的作用机制。例如，一些研究表明，通过调节花生四烯酸代谢途径，可以降低炎症介质的生成，从而减轻炎症反应，预防和治疗炎症性疾病。此外，还有一些研究试图通过抑制花生四烯酸代谢途径，阻止肿瘤细胞的生长和扩散，具有一定的潜在治疗价值。
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