Rat thrombomodulin,TM ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠血栓调节蛋白(TM)酶联免疫试剂盒（货号：CSB-E07939r），采用双抗体夹心法原理进行定量检测，适用于分析血清、血浆、组织匀浆及细胞裂解液等多种样本类型。TM是一种主要表达于血管内皮细胞表面的糖蛋白，参与调节凝血与抗凝平衡，其表达水平与内皮损伤、血栓形成及炎症反应密切相关，是研究血管功能及凝血机制的重要生物标志物。该试剂盒检测灵敏度高，线性范围为1.56-100 ng/mL，通过预包被特异性捕获抗体与标记检测抗体的协同作用，结合HRP酶显色系统实现靶标蛋白的精准定量，操作流程标准化且重复性良好。科研人员可将其应用于心血管疾病模型、药物作用机制、内皮细胞功能研究等领域，例如评估病理状态下TM的表达变化或探究干预措施对凝血调控的影响，为体外实验提供可靠的定量分析工具。本产品仅供科学研究使用。
别名：

N/A
缩写：

TM
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	87-95
1:2	Average %	94
	Range %	89-97
1:4	Average %	99
	Range %	94-102
1:8	Average %	95
	Range %	91-99

回收率：

The recovery of rat TM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-97
EDTA plasma (n=4)	95	92-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.