Pig thrombomodulin,TM ELISA Kit

产品详情

产品描述：

CUSABIO猪血栓调节蛋白（Thrombomodulin, TM）酶联免疫检测试剂盒（货号：CSB-E06530p），采用双抗体夹心法定量检测猪血清、血浆及组织匀浆样本中的TM浓度，检测灵敏度达0.312 ng/mL，线性范围为0.312-20 ng/mL。血栓调节蛋白是血管内皮细胞表面重要的跨膜糖蛋白，通过激活蛋白C系统参与抗凝血调节，同时具有调控炎症反应和细胞保护功能，其表达水平与内皮损伤、血栓形成及炎症性疾病密切相关。本试剂盒包含预包被特异性抗体的酶标板、标准品、检测抗体及显色系统，操作流程标准化，适用于实验室研究猪心血管疾病模型、凝血功能异常机制、炎症相关病理过程及内皮细胞功能评估等领域，可兼容多种生物样本类型，为科研人员提供稳定可靠的TM定量分析工具。
别名：

N/A
缩写：

TM
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig TM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	97
	Range %	92-103
1:4	Average %	85
	Range %	81-87
1:8	Average %	89
	Range %	83-96

回收率：

The recovery of pig TM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	110	103-118

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.