Rat rudimental bovine serum albumin(BSA) check-up ELISA kit

产品详情

产品描述：

CUSABIO基于竞争法原理定量检测大鼠血清、血浆及组织匀浆样本中牛血清白蛋白（BSA）残留的酶联免疫试剂盒。BSA作为细胞培养和生物制剂制备中常用的载体蛋白，其残留可能干扰实验数据准确性，尤其在抗体药物研发、疫苗生产及生物材料研究中需精准控制残留量。试剂盒采用预包被BSA抗体的微孔板结构，通过样本中BSA与标记抗原竞争结合抗体的原理实现定量检测，灵敏度覆盖0.312-80 μg/mL浓度范围，满足痕量检测需求。该产品适用于生物制品生产流程质控、实验动物血清样本中异源蛋白残留分析，以及生物医药研究中BSA清除工艺的优化验证。操作流程包含标准品梯度稀释、样本前处理及显色反应等步骤，配备完整标准品与实验耗材，可在3.5小时内完成96孔板检测。试剂盒严格验证了血浆中常见蛋白（如纤维蛋白原、免疫球蛋白）的交叉反应性，确保检测特异性，为生命科学领域BSA残留监控提供高性价比解决方案。
缩写：

BSA
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 μg/mL-80 μg/mL
灵敏度：

0.312 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat BSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-110
1:2	Average %	95
	Range %	89-106
1:4	Average %	95
	Range %	92-100
1:8	Average %	96
	Range %	92-99

回收率：

The recovery of rat BSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-102
EDTA plasma (n=4)	96	89-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.