Human bovine serum albumin (BSA) ELISA kit

产品详情

产品描述：

CUSABIO人牛血清白蛋白（BSA）酶联免疫试剂盒（货号：CSB-E08633h），采用竞争法定量检测血清、血浆样本中BSA含量，检测灵敏度达0.312 μg/mL，线性范围覆盖0.312-80 μg/mL，适用于科研实验中BSA浓度的精准测定。BSA作为生物实验中常用的蛋白稳定剂和阻断剂，其残留量可能影响免疫检测、细胞培养等实验结果的可靠性，本试剂盒通过预包被特异性抗体，利用样本中BSA与酶标记BSA竞争结合抗体的特性，结合显色反应实现定量分析，可广泛用于实验室评估样本制备过程中BSA的添加浓度、检测实验体系中的外源性BSA干扰，或作为生物制品生产过程中蛋白成分的质控工具。试剂盒提供标准品和预配试剂，操作流程优化，可在4小时内完成96次检测，为科研人员建立标准化实验方案、排除交叉污染提供高效解决方案。
缩写：

BSA
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

0.312 μg/mL-80 μg/mL
灵敏度：

0.312 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human BSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-92
1:2	Average %	95
	Range %	91-105
1:4	Average %	95
	Range %	92-100
1:8	Average %	93
	Range %	85-98

回收率：

The recovery of human BSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-108
EDTA plasma (n=4)	97	90-110

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.