Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

产品详情

产品评价

靶点详情

产品详情

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

大鼠正常T细胞表达和分泌因子/趋化因子5(RANTES/CCL5)酶联免疫试剂盒
货号：

CSB-E07398r-IS
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
相关产品
靶点详情

产品描述：

CUSABIO定量检测大鼠血清、血浆、细胞培养上清及组织匀浆中正常T细胞表达和分泌因子/趋化因子5（RANTES/CCL5）的酶联免疫试剂盒，基于双抗体夹心法原理开发，具有高度特异性。RANTES/CCL5作为CC趋化因子家族成员，在炎症反应、免疫细胞趋化及病毒感染中发挥关键作用，尤其在介导T淋巴细胞和单核细胞迁移的研究中具有重要意义。该试剂盒检测灵敏度为15.6 pg/mL，线性范围覆盖15.6-1000 pg/mL，实验全程约需4小时，操作流程包含标准品梯度稀释、样本孵育、洗板及显色分析，适用于多种基础科研场景：包括但不限于炎症性疾病机制研究（如关节炎模型）、免疫应答动态分析、病毒感染相关趋化因子调控实验（如HIV相关研究），以及药物开发过程中抗炎或免疫调节化合物的体外效果评估。试剂盒提供预包被96孔板、标准品、检测抗体及全套实验缓冲液，兼容常规酶标仪分析，样本无需特殊预处理，适用于批量检测且重复性良好，为探索RANTES/CCL5在病理生理过程中的作用提供可靠工具。
别名：

Ccl5 ELISA Kit; Scya5C-C motif chemokine 5 ELISA Kit; SIS-delta ELISA Kit; Small-inducible cytokine A5 ELISA Kit; T-cell-specific protein RANTES ELISA Kit
缩写：

CCL5
Uniprot No.：

P50231
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat RANTES/CCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:10	Average %	99
	Range %	92-105
1:20	Average %	93
	Range %	89-98
1:40	Average %	101
	Range %	96-106
1:80	Average %	90
	Range %	85-96

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

2.863

2.914

2.888

2.738

500

1.685

1.617

1.651

1.501

250

0.772

0.738

0.755

0.605

125

0.445

0.429

0.437

0.287

62.5

0.313

0.315

0.314

0.164

31.2

0.225

0.233

0.229

0.079

15.6

0.196

0.189

0.192

0.042

0.152

0.148

0.150

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
亚细胞定位：

Secreted.
蛋白家族：

Intercrine beta (chemokine CC) family
数据库链接：

STRING: 10116.ENSRNOP00000014865

UniGene: Rn.8019

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.