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  4. Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

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  • 中文名称：
    大鼠正常T细胞表达和分泌因子/趋化因子5(RANTES/CCL5)酶联免疫试剂盒
  • 货号：
    CSB-E07398r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO大鼠正常T细胞表达和分泌因子/趋化因子5（RANTES/CCL5）酶联免疫检测试剂盒（货号：CSB-E07398r），采用双抗体夹心法定量检测血清、血浆、细胞培养上清及组织匀浆样本中RANTES/CCL5的蛋白水平。RANTES作为CC趋化因子家族的重要成员，主要由活化的T淋巴细胞分泌，通过介导免疫细胞趋化参与炎症反应、自身免疫性疾病及病毒感染等病理生理过程，是免疫调控和炎症机制研究的核心靶标之一。本试剂盒灵敏度高达0.156 ng/mL，线性检测范围为0.156-10 ng/mL，可精准捕获低丰度目标分子，适用于体外实验中对大鼠疾病模型（如关节炎、神经炎症或病毒感染模型）中RANTES动态表达的定量分析，也可用于药物研发过程中评价免疫调节剂或抗炎药物对趋化因子通路的影响。操作流程基于预包被特异性捕获抗体的96孔板，通过生物素标记检测抗体与链霉亲和素-HRP系统实现信号放大，支持科研人员高效完成样本检测与数据获取。
  • 别名：
    Ccl5 ELISA Kit; Scya5C-C motif chemokine 5 ELISA Kit; SIS-delta ELISA Kit; Small-inducible cytokine A5 ELISA Kit; T-cell-specific protein RANTES ELISA Kit
  • 缩写：
    RANTES/CCL5
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat RANTES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 99
    Range % 94-105
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 92
    Range % 88-96
    1:8 Average % 101
    Range % 97-106
  • 回收率：
    The recovery of rat RANTES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 87 83-92
    EDTA plasma (n=4) 92 86-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.684 2.799 2.742 2.633
    5 2.178 2.349 2.264 2.155
    2.5 1.444 1.489 1.467 1.358
    1.25 0.783 0.795 0.789 0.680
    0.625 0.376 0.392 0.384 0.275
    0.312 0.258 0.264 0.261 0.152
    0.156 0.201 0.208 0.205 0.096
    0 0.108 0.109 0.109 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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靶点详情

  • 功能：
    Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 数据库链接：

    STRING: 10116.ENSRNOP00000014865

    UniGene: Rn.8019

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |