Rat procollagen type III (HPCⅢ) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠Ⅲ型前胶原（HPCⅢ）酶联免疫检测试剂盒（货号：CSB-E15810r），基于双抗体夹心法定量原理开发，适用于血清、血浆、细胞培养上清液及组织匀浆样本中HPCⅢ的精准检测。HPCⅢ是胶原代谢的关键前体蛋白，其水平变化与组织纤维化进程密切相关，尤其在肝纤维化、肺纤维化等病理过程中可作为重要生物标志物，为相关疾病机制研究提供实验依据。该试剂盒灵敏度可达到0.156 ng/mL，线性检测范围覆盖0.156 ng/mL至10 ng/mL，可稳定检测微量样本中的目标蛋白。试剂盒包含预包被特异性抗体的酶标板及标准品、检测抗体等核心组分，操作流程标准化，适用于体外实验环境下对纤维化疾病模型、药物干预效果或毒性作用研究中HPCⅢ的动态监测，为科研人员探究组织修复机制及抗纤维化治疗策略提供可靠工具。
别名：

procollagen type 3 ,HPC3|procollagen type III ,HPCⅢ procollagen type III ,HPCIII
缩写：

HPCⅢ
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	84
	Range %	80-88
1:10	Average %	97
	Range %	91-102
1:20	Average %	97
	Range %	92-104
1:40	Average %	93
	Range %	90-96

回收率：

The recovery of rat HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-98
EDTA plasma (n=4)	97	92-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.