Mouse procollagen type III (HPCⅢ) ELISA Kit

中文名称：

小鼠Ⅲ型前胶原（HPCⅢ）酶联免疫试剂盒
货号：

CSB-E17125m
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
引用文献
相关产品

产品详情

产品描述：

小鼠Ⅲ型前胶原（HPCⅢ）酶联免疫试剂盒（CSB-E17125m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的HPCⅢ含量。小鼠Ⅲ型前胶原（HPCⅢ）是细胞外基质（ECM）的重要组成部分，其合成与降解失衡是肝纤维化的关键因素。砷暴露可诱导HPCⅢ表达增加，促进肝星状细胞（HSC）活化，导致ECM过度沉积，进而引发肝纤维化。研究揭示，HPCⅢ在肝纤维化发生发展中扮演着重要角色。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研中纤维化疾病机制或组织修复相关研究。该产品广泛应用于肝纤维化模型、肺纤维化模型或皮肤损伤修复等动物实验研究，为探索HPCⅢ在疾病发生发展中的作用或评价抗纤维化药物效果提供可靠工具，适用于体外实验环境下的浓度测定及动态变化分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

procollagen type 3 ,HPC3|procollagen type III ,HPCⅢ procollagen type III ,HPCIII
缩写：

HPCⅢ
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	97
	Range %	92-101
1:200	Average %	95
	Range %	90-100
1:400	Average %	91
	Range %	85-95
1:800	Average %	102
	Range %	98-107

回收率：

The recovery of mouse HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	84	80-88

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.492	2.343	2.418	2.316
500	1.825	1.716	1.771	1.669
250	0.947	1.004	0.976	0.874
125	0.571	0.582	0.577	0.475
62.5	0.359	0.339	0.349	0.247
31.2	0.246	0.237	0.242	0.140
15.6	0.164	0.171	0.168	0.066
0	0.098	0.105	0.102

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

Farnesoid X receptor activation is required for the anti-inflammatory and anti-oxidative stress effects of Alisol B 23-acetate in carbon tetrachloride-induced liver fibrosis in mice L Zhang,International immunopharmacology,2023
Oxyberberine suppressed the carbon tetrachloride-induced liver fibrosis by inhibiting liver inflammation in a sirtuin 3-dependent manner in mice S Zhao,International Immunopharmacology,2023
SIRT3 Facilitates Amniotic Fluid Stem Cells to Repair Diabetic Nephropathy Through Protecting Mitochondrial Homeostasis by Modulation of Mitophagy Feng J.et al,Cellular Physiology and Biochemistry,2018

Mouse procollagen type III (HPCⅢ) ELISA Kit

产品详情

引用文献

产品评价

相关产品

Most popular with customers

产品中心

信号通路

技术信息

联系信息