Rat oxyntomodulin ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠胃泌酸调节素（OXM）酶联免疫检测试剂盒（货号：CSB-E12949r），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的OXM含量，检测范围为125-8000 pg/mL。胃泌酸调节素是由肠道L细胞分泌的多肽激素，通过调节胃酸分泌、促进胰岛素释放及抑制食欲参与能量代谢平衡，在肥胖、糖尿病等代谢性疾病机制研究中具有重要价值。本试剂盒通过高特异性抗体对实现精准定量，适用于大鼠模型中OXM表达水平与胃肠功能、代谢调控的相关性研究，可应用于药物干预、饮食调控或病理状态下OXM动态变化的科研分析。样本处理简便，标准曲线覆盖生理及病理浓度范围，满足基础研究中血清、血浆等液态样本及胃肠组织匀浆的检测需求，为消化系统与代谢调控领域的分子机制探索提供可靠工具。
缩写：

OXM
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

125 pg/mL-8000 pg/mL
灵敏度：

31.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat oxyntomodulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-92
1:2	Average %	96
	Range %	90-102
1:4	Average %	97
	Range %	92-104
1:8	Average %	93
	Range %	90-96

回收率：

The recovery of rat oxyntomodulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-100
EDTA plasma (n=4)	99	95-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.