Human oxyntomodulin ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测人胃泌酸调节素（OXM）的酶联免疫试剂盒（货号：CSB-E12948h），采用双抗体夹心法原理，适用于检测血清、血浆、细胞培养上清液、尿液及组织匀浆等多种样本类型，检测灵敏度达0.312 ng/mL，线性范围为0.312-20 ng/mL。OXM是一种由肠道L细胞分泌的肽类激素，属于胰高血糖素/胃泌素家族成员，在调节食欲、能量代谢及血糖稳态中发挥重要作用，其浓度变化与肥胖、糖尿病等代谢性疾病密切相关。本试剂盒通过高特异性抗体对实现靶标分子的精准捕获与检测，操作流程标准化，适用于体外研究场景下OXM的表达水平分析，可为能量代谢调控机制研究、胃肠道功能探索及代谢性疾病分子机制研究提供可靠数据支持，满足基础科研与药物研发中对OXM定量检测的需求。
缩写：

OXM
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates, urine, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Oxyntomodulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	83-94
1:2	Average %	84
	Range %	80-89
1:4	Average %	95
	Range %	89-99
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of human Oxyntomodulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	98	92-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.