Rat myelin basic protein(MBP) antibody ELISA Kit

产品详情

产品描述：

CUSABIO大鼠髓鞘碱性蛋白（MBP）抗体定量检测专用酶联免疫试剂盒（货号：CSB-E17399r），通过夹心法原理实现对血清、血浆样本中MBP蛋白的精准检测。MBP是中枢神经系统髓鞘的主要结构蛋白，其表达水平与神经髓鞘形成、损伤修复及多发性硬化等疾病进程密切相关。试剂盒采用双抗体夹心设计，检测范围覆盖0.312 ng/mL至20 ng/mL，灵敏度达0.078 ng/mL，适用于神经退行性疾病模型研究、神经损伤修复机制探索以及药物开发过程中MBP生物标志物的动态监测。操作流程包含标准品预包被板、生物素标记抗体及链霉亲和素-HRP反应体系，可在4小时内完成检测，标准品和样本仅需50μL即可完成分析。本试剂盒提供优化的反应缓冲液和详尽的实验方案，支持科研人员高效开展神经科学基础研究、病理模型评估或神经保护剂筛选等实验，数据稳定性和重复性良好，为揭示MBP在神经生物学中的功能提供可靠工具。
缩写：

MBP Ab
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MBP antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-97
1:2	Average %	95
	Range %	89-99
1:4	Average %	90
	Range %	81-97
1:8	Average %	95
	Range %	91-100

回收率：

The recovery of rat MBP antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.