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中文名称:人髓鞘碱性蛋白(MBP)抗体酶联免疫试剂盒
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货号:CSB-E04787h
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:CUSABIO人髓鞘碱性蛋白(MBP)抗体酶联免疫检测试剂盒(货号CSB-E04787h),基于夹心法原理设计,可对血清或脑脊液样本中的MBP进行定量分析,线性检测范围为0.625-40 ng/mL。MBP是中枢神经系统髓鞘的主要组成蛋白,在维持神经轴突绝缘传导中起关键作用,其异常表达与神经损伤、脱髓鞘疾病等病理过程密切相关。该试剂盒通过双抗体夹心法实现高特异性检测,实验流程标准化,适用于科研领域中对神经系统疾病模型的机制研究、药物干预效果评估或生物标志物探索,例如多发性硬化症、脑创伤或神经退行性疾病的体外实验分析。试剂盒提供预包被微孔板及标准品、检测抗体等核心组分,支持科研人员精确量化样本中MBP的浓度变化,为神经生物学、病理学及药理学研究提供可靠工具。本产品仅限科学研究使用,操作需参照说明书进行样本预处理和标准曲线建立。
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缩写:MBP Ab
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种属:Homo sapiens (Human)
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样本类型:serum, cerebrospinal fluid (CSF)
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检测范围:0.625 ng/mL-40 ng/mL
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灵敏度:0.156 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Neuroscience
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human MBP antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 85-103 1:2 Average % 95 Range % 84-100 1:4 Average % 97 Range % 90-105 1:8 Average % 92 Range % 82-106 -
回收率:
The recovery of human MBP antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 83-99 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 40 2.595 2.658 2.627 2.524 20 2.121 2.136 2.129 2.026 10 1.467 1.473 1.470 1.367 5 0.848 0.896 0.872 0.769 2.5 0.475 0.494 0.485 0.382 1.25 0.311 0.298 0.305 0.202 0.625 0.234 0.253 0.244 0.141 0 0.102 0.104 0.103 -
数据处理:
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货期:3-5 working days
引用文献
- Soluble CD146 in cerebrospinal fluid of active multiple sclerosis Duan H et al,Neuroscience,2013
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