Rat low density lipoprotein,LDL ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠低密度脂蛋白（LDL）酶联免疫检测试剂盒（货号：CSB-E11704r），采用竞争法定量原理，可精准检测大鼠血清、血浆及组织匀浆样本中的LDL水平。LDL是胆固醇运输的主要载体，其异常升高与动脉粥样硬化等心血管疾病密切相关，是脂质代谢研究的重要指标。该试剂盒通过将样本中的LDL与酶标抗原共同竞争结合预包被抗体，经显色反应后，吸光度值与LDL浓度呈负相关，检测灵敏度达0.312 μg/mL，线性范围覆盖0.312-20 μg/mL，满足大鼠模型研究中不同浓度样本的检测需求。该试剂盒适用于心血管疾病机制研究、药物干预效果评价及脂代谢相关实验，例如高脂饲料诱导的动脉硬化模型评估、降脂药物筛选或代谢综合征动物模型中的脂蛋白动态监测。实验操作包含标准品配制、样本预处理、孵育反应及酶标仪检测等步骤，配套提供完整试剂组分与操作指南，确保科研人员高效完成大鼠外周循环或组织局部LDL水平的定量分析。
别名：

N/A
缩写：

LDL
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 μg/mL-20 μg/mL
灵敏度：

0.156 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat LDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	98
	Range %	93-103
1:200	Average %	104
	Range %	100-108
1:400	Average %	87
	Range %	80-94
1:800	Average %	97
	Range %	94-100

回收率：

The recovery of rat LDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	92	88-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.