Mouse low density lipoprotein,LDL ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠低密度脂蛋白（LDL）酶联免疫检测试剂盒（货号：CSB-E13476m），适用于血清、血浆及组织匀浆样本中LDL含量的定量分析。LDL是胆固醇运输的主要载体，其水平异常与动脉粥样硬化等心血管疾病密切相关，该试剂盒为研究脂质代谢机制及疾病模型提供了可靠工具。基于竞争性免疫反应原理，试剂盒通过预包被抗体与样本/标准品中的LDL竞争结合生物素标记抗原，结合辣根过氧化物酶（HRP）标记的链霉亲和素系统实现信号检测，覆盖7.8至500 ng/mL的线性检测范围，满足微量样本的高灵敏度分析需求。该产品可广泛应用于小鼠模型的血脂代谢研究、动脉粥样硬化病理机制探索以及降脂药物或功能性食品的体外药效评估，实验操作全程可在3-4小时内完成。需注意本产品仅限科学研究使用，实验需自备酶标仪、移液器等基础设备。
别名：

N/A
缩写：

LDL
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

7.8 ng/mL-500 ng/mL
灵敏度：

1.95 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	87
	Range %	83-90
1:200	Average %	85
	Range %	81-90
1:400	Average %	95
	Range %	92-98
1:800	Average %	100
	Range %	98-104

回收率：

The recovery of mouse LDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	98	93-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.