Rat keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠角化细胞内分泌因子(KAF)/双调蛋白(AR)酶联免疫检测试剂盒（货号：CSB-E07299r），采用双抗体夹心法原理实现定量分析，适用于检测血清、血浆及组织匀浆样本中的KAF/AR蛋白浓度。KAF/AR属于表皮生长因子家族，在表皮细胞分化、组织修复及炎症调节中发挥重要作用，其表达水平与皮肤屏障功能、上皮再生等生理病理过程密切相关。试剂盒检测范围为1.25-80 pg/mL，可精准检测微量目标蛋白，实验操作全程约需4.5小时，需自备酶标仪及450nm滤光片。该产品为科研领域提供可靠工具，可用于研究皮肤疾病模型、上皮损伤修复机制、慢性炎症反应或肿瘤微环境中KAF/AR的动态变化，特别适用于动物实验中的分子机制探索及生物标志物分析。试剂盒包含预包被捕获抗体的酶标板、检测抗体、标准品及显色底物等核心组分，建议通过标准曲线建立和数据拟合实现样本浓度的准确计算。
别名：

Areg; Sdgf; Amphiregulin; AR; Schwannoma-derived growth factor; SDGF
缩写：

AREG
Uniprot No.：

P24338
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.25 pg/mL-80 pg/mL
灵敏度：

0.31 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	95
	Range %	91-99
1:4	Average %	86
	Range %	80-92
1:8	Average %	96
	Range %	92-102

回收率：

The recovery of rat KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	98-108
EDTA plasma (n=4)	97	92-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
基因功能参考文献：
1. Report increased AREG transcript and protein levels in the lungs of rodents with DA-induced bronchiolitis obliterans. PMID: 24816162
2. estrogen and progeterone increased cell proliferation in normal gland and mammary cancers through the increased production of amphiregulin PMID: 21258428
3. may play a role in the ovulatory process of cycling rats as well as in the induction of ovulation in immature rats PMID: 15291759
4. the expression of amphiregulin (AR), a member of the epidermal growth factor (EGF) family, is rapidly and highly up-regulated by PTH in several osteoblastic cell lines and bone tissues PMID: 15509566
5. findings identify Amphiregulin as a factor for resistance of glioma cells to delta9-Tetrahydrocannabinol induced apoptosis PMID: 19229996
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亚细胞定位：

Membrane; Single-pass membrane protein.
蛋白家族：

Amphiregulin family
数据库链接：

KEGG: rno:29183

STRING: 10116.ENSRNOP00000003703

UniGene: Rn.10568