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中文名称：大鼠1,4,5-三磷酸肌醇(IP3)酶联免疫试剂盒
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货号：CSB-E13004r
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：CUSABIO定量检测大鼠1,4,5-三磷酸肌醇（IP3）的酶联免疫试剂盒（货号CSB-E13004r），采用竞争法检测原理，适用于血清、血浆、组织匀浆、细胞培养上清及细胞裂解液等多种样本类型。IP3作为细胞内重要的第二信使，参与调控钙离子释放、细胞信号转导等过程，在细胞增殖、分化及代谢研究中具有关键作用。试剂盒检测灵敏度为5 pg/mL，线性范围为5-1000 pg/mL，可精准测定样本中IP3的动态变化。该产品通过优化抗体-抗原竞争反应体系，结合酶标板显色分析，实现科研场景下IP3含量的高效检测，适用于神经生物学、心血管疾病模型、药物作用机制等研究领域，为探索细胞内信号通路、评估化合物干预效果提供可靠工具。实验操作全程约需4小时，配套提供标准品及预处理试剂，需自备酶标仪等基础设备。
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缩写：IP3
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种属：Rattus norvegicus (Rat)
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样本类型：serum, plasma, tissue homogenates, cell culture supernates, cell lysates
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检测范围：5 pg/mL-1000 pg/mL
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灵敏度：2.5 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Signal Transduction
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测定原理：quantitative
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测定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 85-96 1:2 Average % 94 Range % 89-98 1:4 Average % 96 Range % 87-102 1:8 Average % 97 Range % 91-104 -
回收率：
The recovery of rat IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 91-105 EDTA plasma (n=4) 95 92-98 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 1000 0.141 0.143 0.142 250 0.270 0.261 0.266 80 0.423 0.440 0.432 20 0.744 0.759 0.752 5 1.156 1.162 1.159 0 2.199 2.214 2.207 -
数据处理：
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货期：3-5 working days
引用文献
- Selective Inhibition of P2Y1 and P2Y12 Receptor Signal Pathways in Platelet Aggregation in Transgenic Cell Lines and Rats by Potassium 2-(1-Hydroxypentyl)-Benzoate, Puerarin and Salvianolic Acid B Y Li, W Wang, J Cai, N Feng, S Xu,CNS Neuroscience & Therapeutics,2024
- Anandamide modulates WNT-5A/BCL-2, IP3/NFATc1, and HMGB1/NF-κB trajectories to protect against mercuric chloride-induced acute kidney injury DM Abdallah,Scientific reports,2023
- Nitric Oxide Linked to mGluR5 Upregulates BDNF Synthesis by Activating MMP2 in the Caudate and Putamen after Challenge Exposure to Nicotine in Rats J Kim,International journal of molecular sciences,2022
- Kaempferol suppresses acetaminophen-induced liver damage by upregulation/activation of SIRT1 Nora Abdullah AlFaris,Pharmaceutical Biology,2021
- Amphetamine-induced conditioned place preference and changes in mGlu1/5 receptor expression and signaling in the rat medial prefrontal cortex Mao LM, et al,Neuroscience,2018
- Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling Ji Eun Kim, et al,International Journal of Molecular Medicine,2018
- Molecular mechanism of Danshensu on platelet antiaggregation Chen Yu.et al,American Institute of Physics,2018
- Alterations in mGlu5 receptor expression and function in the striatum in a rat depression model Mao LM.et al,Journal of Neurochemistry,2018
- Melatonin Promotes Brain-Derived Neurotrophic Factor (BDNF) Expression and Anti-Apoptotic Effects in Neonatal Hemolytic Hyperbilirubinemia via a Phospholipase Yong Luo.et al,Medical Science Monitor 23,2017
- Protective role of Pollen Typhae total flavone against the palmitic acid-induced impairment of glucose-stimulated insulin secretion involving GPR40 signaling in INS-1 cells Feng XT.et al,Int J Mol Med,2017
- Uridine stimulate laxative effect in the loperamide-induced constipation of SD rats through regulation of the mAChRs signaling pathway and mucin secretion Ji Eun Kim,.et al,BMC Genet.,2017
- Effects of five candidate laxatives derived from Liriope platyphylla on the 5-HT receptor signaling pathway in three cell types present in the transverse colon Ji Eun Kim.et al,Molecular Medicine Reports,2016
- A long-acting β2-adrenergic agonist increases the expression of muscarine cholinergic subtype Liu YH.et al,Mol Med Rep.,2015
- A long-acting ß2-adrenergic agonist increases the expression of muscarine cholinergic subtype-3 receptors by activating the ß2-adrenoceptor cyclic adenosine monophosphate signaling pathway in airway smooth muscle cells. Yuan-Hua Liu et al,Molecular Medicine reports,2015
- Lipid peroxidation is essential for phospholipase C activity and IP3 related calcium signal Domijan AM et al,J Cell Sci,2013
- Effect of FSH receptor-binding inhibitor-8 on FSH-mediated granulosa cell signaling and proliferation. Navalakhe RM et al,Chem Biol Drug Des,2013
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